Research Group Bioanalytical Mass Spectrometry

Publications

Journal Article (287)

  1. 1.
    Behler, J.; Sharma, K.; Reimann, V.; Wilde, A.; Urlaub, H.; Hess, W. R.: The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR-Cas subtype III-Bv system. Nature Microbiology (2018)
  2. 2.
    Haselbach, D.; Komarov, I.; Agafonov, D. E.; Hartmuth, K.; Graf, B.; Dybkov, O.; Urlaub, H.; Kastner, B.; Lührmann, R.; Stark, H.: Structure and conformational dynamics of the human spliceosomal Bact complex. Cell 172 (3), pp. 454 - 464 (2018)
  3. 3.
    Buddeweg, A.; Sharma, K.; Urlaub, H.; Schmitz, R. A.: sRNA41 affects ribosome binding sites within polycistronic mRNAs in Methanosarcina mazei Gö1. Molecular Microbiology (2017)
  4. 4.
    Sonnleitner, E.; Wulf, A.; Campagne, S.; Pei, X. Y.; Wolfinger, M. T.; Forlani, G.; Prindl, K.; Abdou, L.; Resch, A.; Allain, F. H. et al.; Luisi, B. F.; Urlaub, H.; Bläsi, U.: Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa. Nucleic Acids Research (2017)
  5. 5.
    Fard, M. K.; van der Meer, F.; Sánchez, P.; Cantuti-Castelvetri, L.; Mandad, S.; Jäkel, S.; Fornasiero, E. F.; Schmitt, S.; Ehrlich, M.; Starost, L. et al.; Kuhlmann, T.; Sergiou, C.; Schultz, V.; Wrzos, C.; Brück, W.; Urlaub, H.; Dimou, L.; Stadelmann, C.; Simons, M.: BCAS1 expression defines a population of early myelinating oligodendrocytes in multiple sclerosis lesions. Science Translational Medicine (2017)
  6. 6.
    Bao, P.; Will, C. L.; Urlaub, H.; Boon, K. L.; Lührmann, R.: The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bact complex. Genes and Development 31 (23-24), pp. 2416 - 2429 (2017)
  7. 7.
    Ahdash, Z.; Lau, A. M.; Byrne, R. T.; Lammens, K.; Stützer, A.; Urlaub, H.; Booth, P. J.; Reading, E.; Hopfner, K. P.; Politis, A.: Mechanistic insight into the assembly of the HerA-NurA helicase-nuclease DNA end resection complex. Nucleic Acids Research 45 (20), pp. 12025 - 12038 (2017)
  8. 8.
    Hillen, H. S.; Parshin, A. V.; Agaronyan, K.; Morozov, Y. I.; Graber, J. J.; Chernev, A.; Schwinghammer, K.; Urlaub, H.; Anikin, M.; Cramer, P. et al.; Temiakov, D.: Mechanism of transcription anti-termination in human mitochondria. Cell 171 (5), pp. 1082 - 1093 (2017)
  9. 9.
    Zaman, U.; Urlaub, H.; Abbasi, A.: Protein profiling of non-model plant Cuminum cyminum by gel-based proteomic approach. Phytochemical Analysis (2017)
  10. 10.
    Schilbach, S.; Hantsche, M.; Tegunov, D.; Dienemann, C.; Wigge, C.; Urlaub, H.; Cramer, P.: Structures of transcription pre-initiation complex with TFIIH and Mediator. Nature 551 (7679), pp. 204 - 206 (2017)
  11. 11.
    Warda, A. S.; Kretschmer, J.; Hackert, P.; Lenz, C.; Urlaub, H.; Höbartner, C.; Sloan, K. E.; Bohnsack, M. T.: Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Reports 18 (11), pp. 2004 - 2014 (2017)
  12. 12.
    Scacioc, A.; Schmidt, C.; Hofmann, T.; Urlaub, H.; Kühnel, K.; Perez-Lara, A.: Structure based biophysical characterization of the PROPPIN Atg18 shows Atg18 oligomerization upon membrane binding. Scientific Reports (2017)
  13. 13.
    Batschkus, S.; Cingoez, G.; Urlaub, H.; Miosge, N.; Kirschneck, C.; Meyer-Marcotty, P.; Lenz, C.: A new albumin-depletion strategy improves proteomic research of gingival crevicular fluid from periodontitis patients. Clinical Oral Investigations (2017)
  14. 14.
    Schmidt , C.; Urlaub, H.: Combining cryo-electron microscopy (cryo-EM) and cross-linking mass spectrometry (CX-MS) for structural elucidation of large protein assemblies. Current Opinion in Structural Biology 46, pp. 157 - 168 (2017)
  15. 15.
    Märtens, B.; Sharma, K.; Urlaub, H.; Bläsi, U.: The SmAP2 RNA binding motif in the 3'UTR affects mRNA stability in the crenarchaeum Sulfolobus solfataricus. Nucleic Acids Research 45 (15), pp. 8957 - 8967 (2017)
  16. 16.
    Wottawa, M.; Naas, S.; Böttger, J.; van Belle, G. J.; Möbius, W.; Revelo, N. H.; Heidenreich, D.; von Ahlen, M.; Zieseniss, A.; Kröhnert, K. et al.; Lutz, S.; Lenz, C.; Urlaub, H.; Rizzoli, S. O.; Katschinski, D. M.: Hypoxia-stimulated membrane trafficking requires T-plastin. Acta Physiologica 221 (1), pp. 59 - 73 (2017)
  17. 17.
    Bertram, K.; Agafonov, D. E.; Dybkov, O.; Haselbach, D.; Leelaram, M. N.; Will, C. L.; Urlaub, H.; Kastner, B.; Lührmann, R.; Stark, H.: Cryo-EM structure of a pre-catalytic human spliceosome primed for activation. Cell (2017)
  18. 18.
    Henning, L. M.; Santos, K. F.; Sticht, J.; Jehle, S.; Lee, C. T.; Wittwer, M.; Urlaub, H.; Stelzl, U.; Wahl, M. C.; Freund, C.: A new role for FBP21 as regulator of Brr2 helicase activity. Nucleic Acids Research 45 (13), pp. 7922 - 7937 (2017)
  19. 19.
    Batschkus, S.; Atanassov, I.; Lenz, C.; Meyer-Marcotty, P.; Cingöz, G.; Kirschneck, C.; Urlaub, H.; Miosge, N.: Mapping the secretome of human chondrogenic progenitor cells with mass spectrometry. Annals of Anatomy 212, pp. 4 - 10 (2017)
  20. 20.
    Xu, Y.; Bernecky, C.; Lee, C. T.; Maier, K. C.; Schwalb, B.; Tegunov, D.; Plitzko, J. M.; Urlaub, H.; Cramer, P.: Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex. Nature Communications (2017)
  21. 21.
    Said, N.; Krupp, F.; Anedchenko, E.; Santos, K. F.; Dybkov, O.; Huang, Y. H.; Lee, C. T.; Loll, B.; Behrmann, E.; Bürger, J. et al.; Mielke, T.; Loerke, J.; Urlaub, H.; Spahn, C. M. T.; Weber, G.; Wahl, M. C.: Structural basis for λN-dependent processive transcription antitermination. Nature Microbiology (2017)
  22. 22.
    Treiber, T.; Treiber, N.; Plessmann, U.; Harlander, S.; Daiss, J. L.; Eichner, N.; Lehmann, G.; Schall, K.; Urlaub, H.; Meister, G.: A compendium of RNA-binding proteins that regulate microRNA biogenesis. Molecular Cell 66 (2), pp. 270 - 284 (2017)
  23. 23.
    Mohr, S.; Doebele, C.; Comoglio, F.; Berg, T.; Beck, J.; Bohnenberger, H.; Alexe, G.; Corso, J.; Ströbel, P.; Wachter, A. et al.; Beissbarth, T.; Schnutgen, F.; Cremer, A.; Haetscher, N.; Göllner, S.; Rouhi, A.; Palmqvist, L.; Rieger, M. A.; Schroeder, T.; Bönig, H.; H; Müller-Tidow, C.; Kuchenbauer, F.; Schütz, E.; Green, A. R.; Urlaub, H.; Stegmaier, K.; Humphries, R. K.; Serve, H.; Oellerich, T.: Hoxa9 and Meis1 cooperatively induce addiction to Syk signaling by suppressing miR-146a in acute myeloid leukemia. Cancer Cell 31 (4), pp. 549 - 562 (2017)
  24. 24.
    Losensky, G.; Jung, K.; Urlaub, H.; Pfeifer, F.; Fröls, S.; Lenz, C.: Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH-LC/MS/MS analysis of planktonic and sessile cells. Proteomics 17 (7), 1600111 (2017)
  25. 25.
    Sidarovich, A.; Will, C. L.; Anokhina, M. M.; Ceballos, J.; Sievers, S.; Agafonov, D. E.; Samatov, T.; Bao, P.; Kastner, B.; Urlaub, H. et al.; Waldmann, H.; Lührmann, R.: Identification of a small molecule inhibitor that stalls splicing at an early step of spliceosome activation. eLife (2017)
  26. 26.
    Bertram, K.; Agafonov, D. E.; Liu, W.; Dybkov, O.; Will, C. L.; Hartmuth, K.; Urlaub, H.; Kastner, B.; Stark, H.; Lührmann, R.: Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature 542 (7641), pp. 318 - 323 (2017)
  27. 27.
    Walter, R.; Pan, K. T.; Doebele, C.; Comoglio, F.; Tomska, K.; Bohnenberger, H.; Young, R. M.; Jacobs, L.; Keller, U.; Bönig, H. et al.; Engelke, M.; Rosenwald, A.; Urlaub, H.; Staudt, L. M.; Serve, H.; Zenz, T.; Oellerich, T.: HSP90 promotes Burkitt lymphoma cell survival by maintaining tonic B cell receptor signaling. Blood 129 (5), pp. 598 - 608 (2017)
  28. 28.
    Göllner, S.; Oellerich, T.; Agrawal-Singh, S.; Schenk, T.; Klein, H. U.; Rohde, C.; Pabst, C.; Sauer, T.; Lerdrup, M.; Tavor, S. et al.; Stölzel, F.; Herold, S.; Ehninger, G.; Köhler, G.; Pan, K. T.; Urlaub, H.; Serve, H.; Dugas, M.; Spiekermann, K.; Vick, B.; Jeremias, I.; Berdel, W. E.; Hansen, K.; Zelent, A.; Wickenhauser, C.; Müller, L. P.; Thiede, C.; Müller-Tidow, C.: Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nature Medicine 23 (1), pp. 69 - 78 (2017)
  29. 29.
    Jakhanwal, S.; Lee, C. T.; Urlaub, H.; Jahn, R.: An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly. EMBO Journal 36, pp. 1788 - 1802 (2017)
  30. 30.
    Pan, K. T.; Chen, C. C.; Urlaub, H.; Khoo, K. H.: Adapting data-independent acquisition for mass spectrometry-based protein site-specific N-glycosylation analysis. Analytical Chemistry 89 (8), pp. 4532 - 4539 (2017)
  31. 31.
    Pozzi, B.; Bragado, L.; Will, C. L.; Mammi, P.; Risso, G.; Urlaub, H.; Lührmann, R.; Srebrow, A.: SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing. Nucleic Acids Research 45 (11), pp. 6729 - 6745 (2017)
  32. 32.
    Callegari, S.; Richter, F.; Chojnacka, K.; Jans, D. C.; Lorenzi, I.; Pacheu-Grau, D.; Jakobs, S.; Lenz, C.; Urlaub, H.; Dudek, J. et al.; Chacinska, A.; Rehling, P.: TIM29 is a subunit of the human carrier translocase required for protein transport. FEBS Letters 590 (23), pp. 4147 - 4158 (2016)
  33. 33.
    Strenzke, N.; Chakrabarti, R.; Al-Moyed, H.; Müller, A.; Hoch, G.; Pangrsic, T.; Yamanbaeva, G.; Lenz, C.; Pan, K. T.; Auge, E. et al.; Geiss-Friedlander, R.; Urlaub, H.; Brose, N.; Wichmann, C.; Reisinger, E.: Hair cell synaptic dysfunction, auditory fatigue and thermal sensitivity in otoferlin Ile515Thr mutants. EMBO Journal 35 (23), pp. 2519 - 2535 (2016)
  34. 34.
    Zhang, J. N.; Michel, U.; Lenz, C.; Friedel, C. C.; Köster, S.; d'Hedouville, Z.; Tönges, L.; Urlaub, H.; Bähr, M.; Lingor, P. et al.; Koch, J. C.: Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration. Scientific Reports (2016)
  35. 35.
    Manno, B.; Oellerich, T.; Schnyder, T.; Corso, J.; Lösing, M.; Neumann, K.; Urlaub, H.; Batista, F. D.; Engelke, M.; Wienands, J.: The Dok-3/Grb2 adaptor module promotes inducible association of the lipid phosphatase SHIP with the BCR in a coreceptor-independent manner. European Journal of Immunology 46 (11), pp. 2520 - 2530 (2016)
  36. 36.
    Cretu, C.; Schmitzova, J.; Ponce-Salvatierra, A.; Dybkov, O.; De Laurentiis, E.; Sharma, K.; Will, C. L.; Urlaub, H.; Lührmann, R.; Pena, V.: Molecular architecture of SF3b and structural consequences of its cancer-related mutations. Molecular Cell 64 (2), pp. 307 - 319 (2016)
  37. 37.
    Martin, S.; Bellora, N.; González-Vallinas, J.; Irimia, M.; Chebli, K.; DeToledo, M.; Raabe, M.; Eyras, E.; Urlaub, H.; Blencowe, B. J. et al.; Tazi, J.: Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum. Journal of Neurochemistry (2016)
  38. 38.
    Hsu, M. F.; Pan, K. T.; Chang, F. Y.; Khoo, K. H.; Urlaub, H.; Cheng, C. F.; Chang, G. D.; Haj, F. G.; Meng, T. C.: S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling. Free Radical Biology and Medicine 99, pp. 199 - 213 (2016)
  39. 39.
    Rauhut, R.; Fabrizio, P.; Dybkov, O.; Hartmuth, K.; Pena, V.; Chari, A.; Kumar, V.; Lee, C. T.; Urlaub, H.; Kastner, B. et al.; Stark, H.; Lührmann, R.: Molecular architecture of the Saccharomyces cerevisiae activated spliceosome. Science 353 (6306), pp. 1399 - 1405 (2016)
  40. 40.
    Justa-Schuch, D.; Silvia-Garcia, M.; Pilla, E.; Engelke, M.; Kilisch, M.; Lenz, C.; Möller, U.; Nakamura, F.; Urlaub, H.; Geiss-Friedlander, R.: DPP9 is a novel component of the N-end rule pathway targeting the tyrosine kinase Syk. eLife (2016)
  41. 41.
    Veit, J.; Sachsenberg, T.; Chernev, A.; Aichler, F.; Urlaub, H.; Kohlbacher, O.: LFQProfiler and RNPxl: Open-source tools for label-free quantification and protein-RNA cross-linking integrated into Proteome Discoverer. Journal of Proteome Research 15 (9), pp. 3441 - 3448 (2016)
  42. 42.
    Agafonov, D. E.; van Santen, M.; Kastner, B.; Dube, P.; Will, C. L.; Urlaub, H.; Lührmann, R.: ATPγS stalls splicing after B complex formation but prior to spliceosome activation. RNA 22 (9), pp. 1329 - 1337 (2016)
  43. 43.
    Narayanan, P.; Sondermann, J.; Rouwette, T.; Karaca, S.; Urlaub, H.; Mitkovski, M.; Gomez-Varela, D.; Schmidt, M.: Native Piezo2 interactomics identifies pericentrin as a novel regulator of Piezo2 in somatosensory neurons. Journal of Proteome Research 15 (8), pp. 2676 - 2687 (2016)
  44. 44.
    Helmke, C.; Raab, M.; Rödel, F.; Matthess, Y.; Oellerich, T.; Mandal, R.; Sanhaji, M.; Urlaub, H.; Rödel, C.; Becker, S. et al.; Strebhardt, K.: Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Research 26 (8), pp. 914 - 934 (2016)
  45. 45.
    Lazzaretti, D.; Veith, K.; Kramer, K.; Basquin, C.; Urlaub, H.; Iron, U.; Bono, F.: The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nature Structural and Molecular Biology 23 (8), pp. 705 - 713 (2016)
  46. 46.
    Gleditzsch, D.; Müller-Esparza, H.; Pausch, P.; Sharma, K.; Dwarakanath, S.; Urlaub, H.; Bange, G.; Randau, L.: Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Research 44 (12), pp. 5872 - 5882 (2016)
  47. 47.
    Seehusen, F.; Kiel, K.; Jottini, S.; Wohlsein, P.; Habierski, H.; Seibel, K.; Vogel, T.; Urlaub, H.; Kollmar, M.; Baumgärtner, W. et al.; Teichmann, U.: Axonopathy in the central nervous system is the hallmark of mice with a novel intragenic null mutation of dystonin. Genetics (2016)
  48. 48.
    Boesler, C.; Rigo, N.; Anokhina, M. M.; Tauchert, M. J.; Agafonov, D. E.; Kastner, B.; Urlaub, H.; Ficner, R.; Will, C. L.; Lührmann, R.: A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity. Nature Communications (2016)
  49. 49.
    Mühlhausen, S.; Findeisen, P.; Plessmann, U.; Urlaub, H.; Kollmar, M.: A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes. Genome Research 26 (7), pp. 945 - 955 (2016)
  50. 50.
    Kühn, J.; Wong, L. E.; Pirkuliyeva, S.; Schulz, K.; Schwiegk, C.; Fünfgeld, K.; Keppler, S.; Batista, F. D.; Urlaub, H.; Habeck, M. et al.; Becker, S.; Griesinger, C.; Wienands, J.: The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation. Science Signaling (2016)
  51. 51.
    Butkevich, E.; Hartig, W.; Nikolov, M.; Erck, C.; Grosche, J.; Urlaub, H.; Schmidt, C. F.; Klopfenstein, D. R.; Chua, J. J. E.: Phosphorylation of FEZ1 by microtubule affinity regulating kinases regulates its function in presynaptic protein trafficking. Scientific Reports (2016)
  52. 52.
    Bareth, B.; Nikolov, M.; Lorenzi, I.; Hildenbeutel, M.; Mick, D. U.; Helbig, C.; Urlaub, H.; Ott, M.; Rehling, P.; Dennerlein, S.: Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Molecular Biology of the Cell 27 (10), pp. 1570 - 1580 (2016)
  53. 53.
    Corso, J.; Pan, K. T.; Walter, R.; Doebele, C.; Mohr, S.; Bohnenberger, H.; Ströbel, P.; Lenz, C.; Slabicki, M.; Hüllein, J. et al.; Comoglio, F.; Rieger, M. A.; Zenz, T.; Wienands, J.; Engelke, M.; Serve, H.; Urlaub, H.; Oellerich, T.: Elucidation of tonic and activated B-cell receptor signaling in Burkitt's lymphoma provides insights into regulation of cell survival. Proceedings of the National Academy of Sciences of the United States of America (2016)
  54. 54.
    Fourmann, J. B.; Dybkov, O.; Agafonov, D. E.; Tauchert, M. J.; Urlaub, H.; Ficner, R.; Fabrizio, P.; Lührmann, R.: The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction. eLife (2016)
  55. 55.
    Kohansalnodehi, M.; Chua, J. J.; Urlaub, H.; Jahn, R.; Czernik, D.: Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis. eLife (2016)
  56. 56.
    Hiragami-Hamada, K.; Sörös, S.; Nikolov, M.; Wilkins, B.; Kreuz, S.; Chen, C.; De La Rosa-Velazquez, I. A.; Zenn, H. M.; Kost, N.; Pohl, W. et al.; Chernev, A.; Schwarzer, D.; Jenuwein, T.; Lorincz, M.; Zimmermann, B.; Walla, P. J.; Neumann, H.; Baubec, T.; Urlaub, H.; Fischle, W.: Dynamic and flexible H3K9me3 bridging via HP1beta dimerization establishes a plastic state of condensed chromatin. Nature Communications (2016)
  57. 57.
    Shao, Y. M.; Richter , H.; Sun, S.; Sharma, K.; Urlaub, H.; Randau, L.: A non-stem-loop CRISPR RNA is processed by dual binding Cas6. Structure 24 (4), pp. 547 - 554 (2016)
  58. 58.
    Khan, N.; Lenz, C.; Binder, L.; Pantakani, D. V. K.; Asif, A. R.: Active and repressive chromatin-associated proteome after MPA treatment and the role of midkine in epithelial monolayer permeability. International Journal of Molecular Sciences 17 (4), 597 (2016)
  59. 59.
    Agafonov, D. E.; Kastner, B.; Dybkov, O.; Hofele, R. V.; Liu, W.; Urlaub, H.; Lührmann, R.; Stark, H.: Molecular architecture of the human U4/U6.U5 tri-snRNP. Science 351 (6280), pp. 1416 - 1420 (2016)
  60. 60.
    Heininger, A. U.; Hackert, P.; Andreou, A. Z.; Boon, K. L.; Memet, I.; Prior, M.; Clancy, A.; Schmidt, B.; Urlaub, H.; Schleiff, E. et al.; Sloan, K. E.; Deckers, M.; Lührmann, R.; Enderlein, J.; Klostermeier, D.; Rehling, P.; Bohnsack, M. T.: Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biology 13 (3), pp. 320 - 330 (2016)
  61. 61.
    Cicek, I. Ö.; Karaca, S.; Brankatschk, M.; Eaton, S.; Urlaub, H.; Shcherbata, H. R.: Hedgehog signaling strength is orchestrated by the mir-310 cluster of microRNAs in response to diet. Genetics (2016)
  62. 62.
    Koch, H.; Raabe, M.; Urlaub, H.; Bindereif, A.; Preußer, C.: The polyadenylation complex of Trypanosoma brucei: Characterization of the functional poly(A) polymerase. RNA Biology 13 (2), pp. 221 - 231 (2016)
  63. 63.
    Kirli, K.; Karaca, S.; Dehne, H. J.; Samwer, M.; Pan, K. T.; Lenz, C.; Urlaub, H.; Görlich, D.: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning. eLife (2015)
  64. 64.
    Absmeier, E.; Wollenhaupt, J.; Mozaffari-Jovin, S.; Becke, C.; Lee, C. T.; Preussner, M.; Heyd, F.; Urlaub, H.; Lührmann, R.; Santos, K. F. et al.; Wahl, M. C.: The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation. Genes and Development 29 (24), pp. 2576 - 2587 (2015)
  65. 65.
    Pleiner, T.; Bates, M.; Trakhanov, S.; Lee, C. T.; Schliep, J. E.; Chug, H.; Böhning, M.; Stark, H.; Urlaub, H.; Görlich, D.: Nanobodies: Site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife (2015)
  66. 66.
    Crisci, C.; Raleff, F.; Bagdul, I.; Raabe, M.; Urlaub, H.; Rain, J. C.; Krämer, A.: Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins. Nucleic Acids Research 43 (21), pp. 10456 - 10473 (2015)
  67. 67.
    Zaman, U.; Richter, F. M.; Hofele, R. V.; Kramer, K.; Sachsenberg, T.; Kohlbacher, O.; Lenz, C.; Urlaub, H.: Dithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions. Molecular and Cellular Proteomics 14 (12), pp. 3196 - 3210 (2015)
  68. 68.
    Sharma, K.; Hrle, A.; Kramer, K.; Sachsenberg, T.; Staals, R. H. J.; Randau, L.; Marchfelder, A.; van der Oost, J.; Kohlbacher, O.; Conti, E. et al.; Urlaub, H.: Analysis of protein–RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry. Methods 89, pp. 138 - 148 (2015)
  69. 69.
    Port, S. A.; Monecke, T.; Dickmanns, A.; Spillner, C.; Hofele, R.; Urlaub, H.; Ficner, R.; Kehlenbach, H.: Structural and functional characterization of CRM1-Nup214 interactions reveals multiple FG-binding sites involved in nuclear export. Cell Reports 13 (4), pp. 690 - 702 (2015)
  70. 70.
    Sakin, V.; Richter, S. M.; Hsiao, H. H.; Urlaub, H.; Melchior, F.: Sumoylation of the GTPase Ran by the RanBP2 SUMO E3 ligase complex. Journal of Biological Chemistry 290 (39), pp. 23598 - 23602 (2015)
  71. 71.
    Boesler, C.; Rigo, N.; Agafonov, D. E.; Kastner, B.; Urlaub, H.; Will, C. L.; Lührmann, R.: Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5‘ splice site. RNA (2015)
  72. 72.
    Diaz, S. A.; Malonoski, A. P.; Susumu, K.; Hofele, R. V.; Oh, E.; Medintz, I. L.: Probing the kinetics of quantum dot-based proteolytic sensors. Analytical and Bioanalytical Chemistry 407 (24), pp. 7307 - 7318 (2015)
  73. 73.
    Salat , D.; Winkler, A.; Urlaub, H.; Gessler, M.: Hey bHLH proteins interact with a FBXO45 containing SCF ubiquitin ligase complex and induce its translocation into the nucleus. PLOS One (2015)
  74. 74.
    Kuvardina, O. N.; Herglotz, J.; Kolodziej, S.; Kohrs, N.; Herkt, S.; Wojcik, B.; Oellerich, T.; Corso, J.; Behrens, K.; Kumar, A. et al.; Hussong, H.; Urlaub, H.; Koch, J.; Serve, H.; Bonig, H.; Stocking, C.; Rieger, M. A.; Lausen, J.: RUNX1 represses the erythroid gene expression program during megakaryocytic differentiation. Blood 125 (23), pp. 3570 - 3579 (2015)
  75. 75.
    Kadavath, H.; Hofele, R. V.; Biernat, J.; Kumar, S.; Tepper, K.; Urlaub, H.; Mandelkow, E.; Zweckstetter, M.: Tau stabilizes microtubules by binding at the interface between tubulin heterodimers. Proceedings of the National Academy of Sciences of the United States of America (2015)
  76. 76.
    Cass, S. D. B.; Haas, K. A.; Stoll, B.; Alkhnbashi, O. S.; Sharma, K.; Urlaub, H.; Backofen, R.; Marchfelder, A.; Bolt, E. L.: The role of Cas8 in type I CRISPR interference. Bioscience Reports (2015)
  77. 77.
    Kost, N.; Kaiser, S.; Ostwal, Y.; Riedel, D.; Stützer, A.; Nikolov, M.; Rathke, C.; Renkawitz-Pohl, R.; Fischle, W.: Multimerization of Drosophila sperm protein Mst77F causes a unique condensed chromatin structure. Nucleic Acids Research 43 (6), pp. 3033 - 3045 (2015)
  78. 78.
    Oellerich, T.; Mohr, S.; Corso, J.; Beck, J.; Döbele, C.; Braun, H.; Cremer, A.; Münch, S.; Wicht, J.; Oellerich, M. F. et al.; Bug, G.; Bohnenberger, H.; Perske, C.; Schütz, E.; Urlaub, H.; Serve, H.: FLT3-ITD and TLR9 use Bruton tyrosine kinase to activate distinct transcriptional programs mediating AML cell survival and proliferation. Blood 125 (12), pp. 1936 - 1947 (2015)
  79. 79.
    Jorrin-Novo, J. V.; Pascual, J.; Sanchez-Lucas, R.; Romero-Rodriguez, M. C.; Rodriguez-Ortega, M. J.; Lenz, C.; Valledor, L.: Fourteen years of plant proteomics reflected in Proteomics: Moving from model species and 2DE-based approaches to orphan species and gel-free platforms. Proteomics 15 (5-6), pp. 1089 - 1112 (2015)
  80. 80.
    Lin, T. Y.; Voronovsky, A.; Raabe, M.; Urlaub, H.; Sander, B.; Golas, M. M.: Dual tagging as an approach to isolate endogenous chromatin remodeling complexes from Saccharomyces cerevisiae. Biochimica et Biophysica Acta - Proteins and Proteomics 1854 (3), pp. 198 - 208 (2015)
  81. 81.
    Timm, T.; Lenz, C.; Merkel, D.; Sadiffo, C.; Grabitzki, J.; Klein, J.; Lochnit, G.: Detection and site localization of phosphorylcholine-modified peptides by NanoLC-ESI-MS/MS using precursor ion scanning and multiple reaction monitoring experiments. Journal of the American Society of Mass Spectrometry 26 (3), pp. 460 - 471 (2015)
  82. 82.
    Wohlgemuth, I.; Lenz, C.; Urlaub, H.: Studying macromolecular complex stoichiometries by peptide-based mass spectrometry. Proteomics 15 (5-6), pp. 862 - 879 (2015)
  83. 83.
    Bohnenberger, H.; Ströbel, P.; Mohr, S.; Corso, J.; Berg, T.; Urlaub, H.; Lenz, C.; Serve, H.; Oellerich, T.: Quantitative mass spectrometric profiling of cancer-cell proteomes derived from liquid and solid tumors. Journal of Visualized Experiments (2015)
  84. 84.
    De, I.; Bessonov, S.; Hofele, R.; Dos Santos, K.; Will, C. L.; Urlaub, H.; Lührmann, R.; Pena, V.: The RNA helicase ​Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes. Nature Structural and Molecular Biology (2015)
  85. 85.
    Lugassy, J.; Corso, J.; Beach, D.; Petrik, T.; Oellerich, T.; Urlaub, H.; Yablonski, D.: Modulation of TCR responsiveness by the Grb2-family adaptor, Gads. Cellular Signalling 27 (1), pp. 125 - 134 (2015)
  86. 86.
    Hrle, A.; Maier, L. K.; Sharma, K.; Ebert, J.; Basquin, C.; Urlaub, H.; Marchfelder, A.; Conti, E.: Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family. RNA Biology (2014)
  87. 87.
    Petrova, D. T.; Schultze, F. C.; Brandhorst, G.; Luchs, K. D.; Lenz, C.; Urlaub, H.; Rubel, D.; Gross, O.; Walson, P. D.; Oellerich, M.: Effects of mycophenolate mofetil on kidney function and phosphorylation status of renal proteins in Alport COL4A3-deficient mice. Proteome Science (2014)
  88. 88.
    Staals, R. H.; Zhu, Y.; Taylor, D. W.; Kornfeld, J. E.; Sharma, K.; Barendregt, A.; Koehorst, J. F.; Vlot, M.; Neupane, N.; Varossieau, K. et al.; Sakamoto, K.; Suzuki, T.; Dohmae, N.; Yokoyama, S.; Schaap, P. J.; Urlaub, H.; Heck, A. J. R.; Nogales, E.; Doudna, J. A.; Shinkai, A.; van der Oost, J.: RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Molecular Cell 56 (4), pp. 518 - 530 (2014)
  89. 89.
    Kramer, K.; Sachsenberg, T.; Beckmann, B. M.; Qamar, S.; Boon, K. L.; Hentze, M. W.; Kohlbacher, O.; Urlaub, H.: Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins. Nature Methods 11 (10), pp. 1064 - 1070 (2014)
  90. 90.
    Lenz, C.; Urlaub, H.: Separation methodology to improve proteome coverage depth. Expert Review of Proteomics 11 (4), pp. 409 - 414 (2014)
  91. 91.
    Moritz, B.; Lilie, H.; Naarmann-de Vries, I. S.; Urlaub, H.; Wahle, E.; Ostareck-Lederer, A.; Ostareck, D. H.: Biophysical and biochemical analysis of hnRNP K: Arginine methylation, reversible aggregation and combinatorial binding to nucleic acids. Biological Chemistry 395 (7-8), pp. 837 - 853 (2014)
  92. 92.
    Wilhelm, B. G.; Mandad, S.; Truckenbrodt, S.; Kröhnert, K.; Schäfer, C.; Rammner, B.; Koo, S. J.; Classen, G. A.; Krauss, M.; Haucke, V. et al.; Urlaub, H.; Rizzoli, S. O.: Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science 344 (6187), pp. 1023 - 1028 (2014)
  93. 93.
    Vazquez-Pianzola, P.; Adam, J.; Haldemann, D.; Hain, D.; Urlaub, H.; Suter, B.: Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D. Development 141 (9), pp. 1915 - 1926 (2014)
  94. 94.
    Loedige, I.; Stotz, M.; Qamar, S.; Kramer, K.; Hennig, J.; Schubert, T.; Löffler, P.; Längst, G.; Merkl, R.; Urlaub, H. et al.; Meister, G.: The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation. Genes and Development 28 (7), pp. 749 - 764 (2014)
  95. 95.
    Plagens, A.; Tripp, V.; Daume, M.; Sharma, K.; Klingl, A.; Hrle, A.; Conti, E.; Urlaub, H.; Randau, L.: In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex. Nucleic Acids Research 42 (8), pp. 5125 - 5138 (2014)
  96. 96.
    Brendel, J.; Stoll, B.; Lange, S. J.; Sharma, K.; Lenz, C.; Stachler, A. E.; Maier, L. K.; Richter, H.; Nickel, L.; Schmitz, R. A. et al.; Randau, L.; Allers, T.; Urlaub, H.; Backofen, R.; Marchfelder, A.: A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii. Journal of Biological Chemistry 289 (10), pp. 7164 - 7177 (2014)
  97. 97.
    Schmidt, C.; Groenborg, M.; Deckert, J.; Bessonov, S.; Conrad, T.; Lührmann, R.; Urlaub, H.: Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count. RNA 20 (3), pp. 406 - 420 (2014)
  98. 98.
    Biesemann, C.; Groenborg, M.; Luquet, E.; Wichert, S. P.; Bernard, V.; Bungers, S. R.; Cooper, B.; Varoqueaux, F.; Li, L.; Byrne, J. A. et al.; Urlaub, H.; Jahn, O.; Brose, N.; Herzog, E.: Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO Journal 33 (2), pp. 157 - 170 (2014)
  99. 99.
    Xiang, S. Q.; Gapsys, V.; Kim, H. Y.; Bessonov, S.; Hsiao, H. H.; Möhlmann, S.; Klaukien, V.; Ficner, R.; Becker, S.; Urlaub, H. et al.; Lührmann, R.; de Groot, B.; Zweckstetter, M.: Phosphorylation drives a dynamic switch in serine/arginine-rich proteins. Structure 21 (12), pp. 2162 - 2174 (2013)
 
Go to Editor View
loading content