Guest Group Biomolecular Spectroscopy and Single Molecule Detection


Journal Article (43)

  1. 1.
    Schroeder, S.; Kaufmann, J. D.; Grunwald, M.; Walla, P. J.; Lakomek, N. A.; Wingfield, P. T.: HIV-1 gp41 transmembrane oligomerization monitored by FRET and FCS. FEBS Letters (2018)
  2. 2.
    Pieper, A.; Hohgardt, M.; Willich, M.; Gacek, D. A.; Hafi, N.; Pfennig, D.; Albrecht, A.; Walla, P. J.: Biomimetic light-harvesting funnels for re-directioning of diffuse light. Nature Communications (2018)
  3. 3.
    Walla, P. J.: Photosynthesis: Nature's power switching station. Nature Chemistry 9, pp. 728 - 730 (2017)
  4. 4.
    Gacek, D. A.; Moore, A. L.; Moore, T. A.; Walla, P. J.: Two-photon spectra of chlorophylls and carotenoid–tetrapyrrole dyads. The Journal of Physical Chemistry B 121 (43), pp. 10055 - 10063 (2017)
  5. 5.
    Lin, C. C.; Hsu, H. F.; Walla, P. J.: A one donor–two acceptor lipid bilayer FRET assay based on asymmetrically labeled liposomes. Journal of Physical Chemistry B 120 (43), pp. 11085 - 11092 (2016)
  6. 6.
    Hiragami-Hamada, K.; Sörös, S.; Nikolov, M.; Wilkins, B.; Kreuz, S.; Chen, C.; De La Rosa-Velazquez, I. A.; Zenn, H. M.; Kost, N.; Pohl, W. et al.; Chernev, A.; Schwarzer, D.; Jenuwein, T.; Lorincz, M.; Zimmermann, B.; Walla, P. J.; Neumann, H.; Baubec, T.; Urlaub, H.; Fischle, W.: Dynamic and flexible H3K9me3 bridging via HP1beta dimerization establishes a plastic state of condensed chromatin. Nature Communications (2016)
  7. 7.
    Hafi, N.; Grunwald, M.; van den Heuvel, L. S.; Aspelmeier, T.; Steinem, C.; Korte, M.; Munk, A.; Walla, P. J.: Reply to "Polarization modulation adds little additional information to super-resolution fluorescence microscopy". Nature Methods 13 (1), pp. 8 - 9 (2016)
  8. 8.
    Park, Y.; Seo, J. B.; Fraind, A.; Perez-Lara, A.; Yavuz, H.; Han, K.; Jung, S. R.; Kattan, I.; Walla, P. J.; Choi, M. et al.; Cafiso, D. S.; Koh, D. S.; Jahn, R.: Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength. Nature Structural and Molecular Biology 22 (10), pp. 815 - 823 (2015)
  9. 9.
    Chen, J.; Kellner, Y.; Zagrebelsky, M.; Grunwald, M.; Korte, M.; Walla, P. J.: Two-photon correlation spectroscopy in single dendritic spines reveals fast actin filament reorganization during activity-dependent growth. PLoS One (2015)
  10. 10.
    Holleboom, C. P.; Gacek, D. A.; Liao, P. N.; Negretti, M.; Croce, R.; Walla, P. J.: Carotenoid-chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29. Photosynthesis Research 124 (2), pp. 171 - 180 (2015)
  11. 11.
    Lin, C. C.; Seikowski, J.; Perez-Lara, A.; Jahn, R.; Höbartner, C.; Walla, P. J.: Control of membrane gaps by ​synaptotagmin-Ca2+ measured with a novel membrane distance ruler. Nature Communications (2014)
  12. 12.
    Park, Y.; Vennekate, W.; Yavuz, H.; Preobraschenski, J.; Hernandez, J. M.; Riedel, D.; Walla, P. J.; Jahn, R.: alpha-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery. Journal of Biological Chemistry 289 (23), pp. 16326 - 16335 (2014)
  13. 13.
    Hafi, N.; Grunwald, M.; van den Heuvel, L. S.; Aspelmeier, T.; Chen, J.; Zagrebelsky, M.; Schütte, O. M.; Steinem, C.; Korte, M.; Munk, A. et al.; Walla, P. J.: Fluorescence nanoscopy by polarization modulation and polarization angle narrowing. Nature Methods 11 (5), pp. 579 - 584 (2014)
  14. 14.
    Holleboom, C. P.; Walla, P. J.: The back and forth of energy transfer between carotenoids and chlorophylls and its role in the regulation of light harvesting. Photosynthesis Research 119 (1-2), pp. 215 - 221 (2014)
  15. 15.
    Holleboom, C. P.; Yoo, S.; Liao, P. N.; Compton, I.; Haase, W.; Kirchhoff, H.; Walla, P. J.: Carotenoid-chlorophyll coupling and fluorescence quenching correlate with protein packing density in grana-thylakoids. The Journal of Physical Chemistry B 117 (38), pp. 11022 - 11030 (2013)
  16. 16.
    Wilk, L.; Grunwald, M.; Liao, P. N.; Walla, P. J.; Kühlbrandt, W.: Direct interaction of the major light-harvesting complex II and PsbS in nonphotochemical quenching. Proceedings of the National Academy of Sciences of the United States of America 110 (14), pp. 5452 - 5456 (2013)
  17. 17.
    Munari, F.; Soeroes, S.; Zenn, H. M.; Schomburg, A.; Kost, N.; Schröder, S.; Klingberg, R.; Rezaei-Ghaleh, N.; Stützer, A.; Gelato, K. A. et al.; Walla, P. J.; Becker, S.; Schwarzer, D.; Zimmermann, B.; Fischle, W.; Zweckstetter, M.: Methylation of K9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1β with the nucleosome. Journal of Biological Chemistry 287 (40), pp. 33756 - 33765 (2012)
  18. 18.
    Vennekate, W.; Schröder, S.; Lin, C. C.; van den Bogaart, G.; Grunwald, M.; Jahn, R.; Walla, P. J.: Cis- and trans-membrane interactions of synaptotagmin-1. Proceedings of the National Academy of Siences of the United States of America 109 (27), pp. 11037 - 11042 (2012)
  19. 19.
    Hernandez, J. M.; Stein, A.; Behrmann, E.; Riedel, D.; Cypionka, A.; Farsi, Z.; Walla, P. J.; Raunser, S.; Jahn, R.: Membrane fusion intermediates via directional and full assembly of the SNARE complex. Science 336 (6088), pp. 1581 - 1584 (2012)
  20. 20.
    Liao, P. N.; Pillai, S.; Kloz, M.; Gust, D.; Moore, A. L.; Moore, T. A.; Kennis, J. T. M.; van Grondelle, R.; Walla, P. J.: On the role of excitonic interactions in carotenoid-phthalocyanine dyads and implications for photosynthetic regulation. Photosynthesis Research 111 (1-2), pp. 237 - 243 (2012)
  21. 21.
    Ilioaia, C.; Johnson, M. P.; Liao, P. N.; Pascal, A. A.; van Grondelle, R.; Walla, P. J.; Ruban, A. A.; Robert, B.: Photoprotection in plants involves a change in Lutein 1 binding domain in the major light-harvesting complex of photosystem II. Journal of Biological Chemistry 286 (31), pp. 27247 - 27254 (2011)
  22. 22.
    Liao, P. N.; Pillai, S.; Gust, D.; Moore, T.; Moore, A.; Walla, P. J.: Two-photon study on the electronic interactions between the first excited singlet states in carotenoid-tetrapyrrole dyads. Journal of Physical Chemistry A 115 (16), pp. 4082 - 4091 (2011)
  23. 23.
    Liao, P. N.; Holleboom, C. P.; Wilk, L.; Kuehlbrandt, W.; Walla, P. J.: Correlation of Car S1 -> Chl with Chl -> Car S1 energy transfer supports the excitonic model in quenched light harvesting complex II. The Journal of Physical Chemistry B 114 (47), pp. 16650 - 16655 (2010)
  24. 24.
    Wohlgemuth, I.; Pohl, W.; Rodnina, M. V.: Optimization of speed and accuracy of decoding in translation. The EMBO Journal 29 (21), pp. 3701 - 3709 (2010)
  25. 25.
    Liao, P. N.; Bode, S.; Wilk, L.; Hafi, N.; Walla, P. J.: Correlation of electronic carotenoid-chlorophyll interactions and fluorescence quenching with the aggregation of native LHC II and chlorophyll deficient mutants. Journal of Chemical Physics 373 (1-2), pp. 50 - 55 (2010)
  26. 26.
    Walla, P. J.: Regulation der Photosynthese: Gangschaltung für die Speicherung solarer Energie. Chemie in unserer Zeit 44 (1), pp. 66 - 67 (2010)
  27. 27.
    Hippchen, H.; Pohl, W.; Walla, P. J.: Single-particle identification of encoded nanospheres. Journal of Biomolecular Screening 15 (2), pp. 218 - 223 (2010)
  28. 28.
    Cypionka, A.; Stein, A.; Hernandez, M.; Hippchen, H.; Jahn, R.; Walla, P. J.: Discrimination between docking and fusion of liposomes reconstituted with neuronal SNARE-proteins using FCS. Proceedings of the National Academy of Sciences of the United States of America 106 (44), pp. 18575 - 18580 (2009)
  29. 29.
    Bode, S.; Quentmeier, C.C.; Liao, P.; Haft, N.; Barros, T.; Wilk, L.; Bittner, F.; Walla, P. J.: On the regulation of photosynthesis by excitonic interactions between carotenoids and chlorophylls. Proceedings of the National Academy of Sciences of the United States of America 106 (28), pp. 12311 - 12316 (2009)
  30. 30.
    Quentmeier, S.; Quentmeier, C. C.; Walla, P. J.; Gericke, K. H.: Two-color two-photon excitation of intrinsic protein fluorescence: label-free observation of proteolytic digestion of bovine serum albumin. ChemPhysChem 10, 10.1002/cphc.200800586, pp. 1607 - 1613 (2009)
  31. 31.
    Bode, S.; Quentmeier, C. C.; Liao, P. N.; Barros, T.; Walla, P. J.: Xanthophyll-cycle dependence of the energy transfer between carotenoid dark states and chlorophylls in NPQ mutants of living plants and in LHC II. Chemical Physics Letters 450 (4-6), pp. 379 - 385 (2008)
  32. 32.
    Wehling, A.; Pohl, W.; Gerke, B.; Kipp, S.; Walla, P. J.: Generation of nanopores down to 10 nm for use in deep-nulling interferometry. ChemPhysChem 9 (2), pp. 327 - 331 (2008)
  33. 33.
    Quentmeier, C. C.; Wehling, A.; Walla, P. J.: A bioassay based on the ultrafast response of a reporter molecule. Journal of Biomolecular Screening 12 (3), pp. 341 - 350 (2007)
  34. 34.
    Zwilling, D.; Cypionka, A.; Pohl, W.; Fasshauer, D.; Walla, P. J.; Wahl, M. C.; Jahn, R.: Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies. The EMBO Journal 26 (1), pp. 9 - 18 (2007)
  35. 35.
    Wehling, A.; Walla, P. J.: A two-photon excitation study on the role of carotenoid dark states in the regulation of plant photosynthesis. Photosynthesis Research 90 (2), pp. 101 - 110 (2006)
  36. 36.
    Fraehmcke, J. S.; Walla, P. J.: Coulombic couplings between pigments in the major light-harvesting complex LHC II calculated by the transition density cube method. Chemical Physics Letters 430 (4-6), pp. 397 - 403 (2006)
  37. 37.
    Pohl, W.; Hellmuth, H.; Hilbert, M.; Seibel, J.; Walla, P. J.: A two-photon fluorescence correlation study of lectins interacting with carbohydrated 20nm beads. ChemBioChem 7, pp. 268 - 274 (2006)
  38. 38.
    Wehling, A.; Walla, P. J.: Time-resolved two-photon spectroscopy of photosystem I determines hidden carotenoid dark-state dynamics. Journal of Physical Chemistry B 109 (51), pp. 24510 - 24516 (2005)
  39. 39.
    Hilbert, M.; Hippchen, H.; Wehling, A.; Walla, P. J.: Correlational analysis of proteins and nonmetallic nanoparticles in a deep-nulling microscope. Journal of Physical Chemistry B 109 (38), pp. 18162 - 18170 (2005)
  40. 40.
    Walla, P. J.; Nickel, B.: Intramolecular proton transfer and tunnelling reactions of 14 hydroxyphenylbenzoxazole derivatives in Xenon at 15 K. Chemical Physics 312 (1-3), pp. 177 - 185 (2005)
  41. 41.
    Hilbert, M.; Wehling, A.; Schlodder, E.; Walla, P. J.: Two-photon-sensitized fluorescence and excitation spectra of photosystem I of Thermosynechococcus elongatus. Journal of Physical Chemistry B 108 (34), pp. 13022 - 13030 (2004)
  42. 42.
    Nickel, B.; Walla, P. J.: Anomaly of triplet-triplet energy transfer from 2-(2′-hydroxyphenyl)benzoxazole and similar compounds exhibiting intramolecular proton transfer. Chemical Physics 237, pp. 371 - 394 (1998)
  43. 43.
    Nickel, B.; Grellmann, K. H.; Stephan, J.; Walla, P. J.: Keto-enol tautomerism in the triplet state of hydroxyphenylbenzoxazoles in an alkane glass: Hydrogen tunneling and isotope effects down to 2 K. Berichte der Bunsen-Gesellschaft für Physikalische Chemie 102, pp. 436 - 447 (1998)

Book (2)

  1. 44.
    Walla, P. J. (Ed.): Modern Biophysical Chemistry: Detection and Analysis of Biomolecules. Wiley-VCH, Weinheim (2014), XIV, 344 pp.
  2. 45.
    Walla, P. J.: Modern biophysical chemistry. Wiley-VCH, Weinheim (2009), 301pp. pp.

Book Chapter (2)

  1. 46.
    Walla, P. J.; Holleboom, C. P.; Fleming, G. R.: Electronic carotenoid-chlorophyll interactions regulating photosynthetic llght harvesting of higher plants and green algae. In: Non-Photochemical Quenching and Energy Dissipation in Plants, Algae and Cyanobacteria (Eds. Demmig-Adams, B.; Garab, G.; Adams, W.; Govindjee). Springer, Dordrecht (2014)
  2. 47.
    Haupts, U.; Hesse, O.; Strerath, M.; Walla, P. J.; Coco, W. M.: Confocal and conventional fluorescence-based high throughput screening in protein engineering. In: Protein engineering handbook. Vol. 2, pp. 713 - 748 (Eds. Lutz, S.; Bornscheuer, U. T.). Wiley-VCH, Weinheim (2008)
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