Zeitschriftenartikel (78)

  1. 1.
    Aksu, M.; Pleiner, T.; Karaca, S.; Kappert, C.; Dehne, H. J.; Seibel, K.; Urlaub, H.; Bohnsack, M. T.; Görlich, D.: Xpo7 is a broad-spectrum exportin and a nuclear import receptor. Journal of Cell Biology 217 (7), S. 2329 - 2340 (2018)
  2. 2.
    Frey, S.; Rees, R.; Schünemann, J.; Ng, S. C.; Fünfgeld, K.; Huyton, T.; Görlich, D.: Surface properties determining passage rates of proteins through nuclear pores. Cell 174 (1), S. 202 - 217 (2018)
  3. 3.
    Alexeeva, M.; Guragain, P.; Tesfahun, A. N.; Tomkuviene, M.; Arshad, A.; Gerasimaite, R.; Rugenaite, A.; Urbanaviciute, G.; Bjoras, M.; Laerdahl, J. K. et al.; Klungland, A.; Klimasauskas, S.; Bjelland, S.: Excision of the doubly methylated base N-4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins. Philosophical Transactions of the Royal Society B: Biological Sciences 373 (1748), 20170337 (2018)
  4. 4.
    Abayev, M.; Rodrigues, J. P. G. L. M.; Srivastava, G.; Arshava, B.; Jaremko, L.; Jaremko, M.; Naider, F.; Levitt, M.; Anglister, J.: The solution structure of monomeric CCL5 in complex with a doubly sulfated N-terminal segment of CCR5. FEBS Journal (2018)
  5. 5.
    Celik, A. A.; Simper, G. S.; Huyton, T.; Blasczyk, R.; Bade-Döding, C.: HLA-G mediated immune regulation is impaired by a single amino acid exchange in the alpha 2 domain. Human Immunology 79 (6), S. 453 - 462 (2018)
  6. 6.
    Ananth, A.; Genua, M.; Aissaoui, N.; Diaz, L.; Eisele, N. B.; Frey, S.; Dekker, C.; Richter, R. P.; Görlich, D.: Reversible immobilization of proteins in sensors and solid-state nanopores. Small 14 (18), 1703357 (2018)
  7. 7.
    Bucevicius, J.; Lukinavicius, G.; Gerasimaite, R.: The use of Hoechst dyes for DNA staining and beyond. Chemosensors (2018)
  8. 8.
    Pleiner, T.; Bates, M.; Görlich, D.: A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies. Journal of Cell Biology 217 (3), S. 1143 - 1154 (2018)
  9. 9.
    Ananth, A. N.; Mishra, A.; Frey, S.; Dwarkasing, A.; Versloot, R.; van der Giessen, E.; Görlich, D.; Onck, P.; Dekker, C.: Spatial structure of disordered proteins dictates conductance and selectivity in Nuclear Pore Complex mimics. eLife (2018)
  10. 10.
    Karabinos, A.; Schünemann, J.; Parry, D. A. D.: Assembly studies of six intestinal intermediate filament (IF) proteins B2, C1, C2, D1, D2, and E1 in the nematode C-elegans. Cytoskeleton 74 (3), S. 107 - 113 (2017)
  11. 11.
    Göttfert, F.; Pleiner, T.; Heine, J.; Westphal, V.; Görlich, D.; Sahl, S.; Hell, S. W.: Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proceedings of the National Academy of Sciences of the United States of America (2017)
  12. 12.
    Warda, A. S.; Freytag, B.; Haag, S.; Sloan, K. E.; Görlich, D.; Bohnsack, M.: Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment. Human Molecular Genetics 25 (24), S. 5353 - 5364 (2016)
  13. 13.
    Seehusen, F.; Kiel, K.; Jottini, S.; Wohlsein, P.; Habierski, H.; Seibel, K.; Vogel, T.; Urlaub, H.; Kollmar, M.; Baumgärtner, W. et al.; Teichmann, U.: Axonopathy in the central nervous system is the hallmark of mice with a novel intragenic null mutation of dystonin. Genetics (2016)
  14. 14.
    Kühn, J.; Wong, L. E.; Pirkuliyeva, S.; Schulz, K.; Schwiegk, C.; Fünfgeld, K.; Keppler, S.; Batista, F. D.; Urlaub, H.; Habeck, M. et al.; Becker, S.; Griesinger, C.; Wienands, J.: The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation. Science Signaling (2016)
  15. 15.
    Aksu, M.; Trakhanov, S.; Görlich, D.: Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A. Nature Communications (2016)
  16. 16.
    Zahn, R.; Osmanovic, D.; Ehret, S.; Callis, C. A.; Frey, S.; Stewart, M.; You, C. J.; Görlich, D.; Hoogenboom, B. W.; Richter, R. P.: A physical model describing the interaction of nuclear transport receptors with FG nucleoporin domain assemblies. eLife (2016)
  17. 17.
    Schmidt, H. B.; Görlich, D.: Transport selectivity of nuclear pores, phase separation, and membraneless organelles. Trends in Biochemical Sciences 41 (1), S. 46 - 61 (2016)
  18. 18.
    Kirli, K.; Karaca, S.; Dehne, H. J.; Samwer, M.; Pan, K. T.; Lenz, C.; Urlaub, H.; Görlich, D.: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning. eLife (2015)
  19. 19.
    Pleiner, T.; Bates, M.; Trakhanov, S.; Lee, C. T.; Schliep, J. E.; Chug, H.; Böhning, M.; Stark, H.; Urlaub, H.; Görlich, D.: Nanobodies: Site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife (2015)
  20. 20.
    Chug, H.; Trakhanov, S.; Hülsmann, B. B.; Pleiner, T.; Görlich, D.: Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Science 350 (6256), S. 106 - 110 (2015)
  21. 21.
    Frey, S.; Görlich, D.: The Xenopus laevis Atg4B protease: Insights into substrate recognition and application for tag removal from proteins expressed in pro- and eukaryotic hosts. PLOS One (2015)
  22. 22.
    Schmidt, H. B.; Görlich, D.: Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity. eLife (2015)
  23. 23.
    Migliorini, E.; Thakar, D.; Sadir, R.; Pleiner, T.; Baleux, F.; Lortat-Jacob, H.; Coche-Guerente, L.; Richter, R. P.: Well-defined biomimetic surfaces to characterize glycosaminoglycan-mediated interactions on the molecular, supramolecular and cellular levels. Biomaterials 35 (32), S. 8903 - 8915 (2014)
  24. 24.
    Hattangadi, S. M.; Martinez-Morilla, S.; Patterson, H. C.; Shi, J.; Burke, K.; Avila-Figueroa, A.; Venkatesan, S.; Wang, J.; Paulsen, K.; Görlich, D. et al.; Murata-Hori, M.; Lodish, H. F.: Histones to the cytosol: Exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood (2014)
  25. 25.
    Karabinos, A.; Schünemann, J.: Unusual ultrastructures of the Branchiostoma IF protein C2 containing heptads in the tail. Protoplasma 251 (4), S. 985 - 988 (2014)
  26. 26.
    Frey, S.; Görlich, D.: A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins. Journal of Chromatography A 1337, S. 95 - 105 (2014)
  27. 27.
    Frey, S.; Görlich, D.: Purification of protein complexes of defined subunit stoichiometry using a set of orthogonal, tag-cleaving proteases. Journal of Chromatography A 1337, S. 106 - 115 (2014)
  28. 28.
    Eisele, N. B.; Labokha, A.; Frey, S.; Görlich, D.; Richter , R. P.: Cohesiveness tunes assembly and morphology of FG nucleoporin domain meshworks - Implications for nuclear pore permeability. Biophysical Journal 105 (8), S. 1860 - 1870 (2013)
  29. 29.
    Samwer, M.; Dehne, H. J.; Spira, F.; Kollmar, M.; Gerlich, D.; Urlaub, H.; Görlich, D.: The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis. EMBO Journal 32 (13), S. 1886 - 1902 (2013)
  30. 30.
    Aggarwal, S.; Snaidero, N.; Pähler, G.; Frey, S.; Sanchez, P.; Zweckstetter, M.; Janshoff, A.; Schneider, A.; Weil, M. T.; Schaap, I. A. T. et al.; Görlich, D.; Simons, M.: Myelin membrane assembly is driven by a phase transition of myelin basic proteinsiInto a cohesive protein meshwork. PLoS Biology (2013)
  31. 31.
    Labokha, A.; Gradmann, S.; Frey, S.; Hülsmann, B. B.; Urlaub, H.; Baldus, M.; Görlich, D.: Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes. EMBO Journal 32 (2), S. 204 - 218 (2013)
  32. 32.
    Karabinos, A.; Schünemann, J.; Parry, D. A. D.: A rod domain sequence in segment 1B triggers dimerisation of the two small Branchiostoma IF proteins B2 and A3. European Journal of Cell Biology 91 (10), S. 800 - 808 (2012)
  33. 33.
    Hülsmann, B. B.; Labokha, A.; Görlich, D.: The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. Cell 150 (4), S. 738 - 751 (2012)
  34. 34.
    Petri, M.; Frey, S.; Menzel, A.; Görlich, D.; Techert, S.: Structural characterization of nanoscale meshworks within a nucleoporin FG hydrogel. Biomacromolecules 13 (6), S. 1882 - 1889 (2012)
  35. 35.
    Eisele, N. B.; Andersson, F. I.; Frey, S.; Richter, R. P.: Viscoelasticity of thin biomolecular films: A case study on nucleoporin phenylalanine-glycine repeats grafted to a histidine- tag capturing QCM-D sensor. Biomacromolecules 13 (8), S. 2322 - 2332 (2012)
  36. 36.
    Aggarwal, S.; Yurlova, L.; Snaidero, N.; Reetz, C.; Frey, S.; Zimmermann, J.; Pahler, G.; Janshoff, A.; Friedrichs, J.; Mueller, D. J. et al.; Goebel, C.; Simons, M.: A size barrier limits protein diffusion at the cell surface to generate lipid-rich myelin-membrane sheets. Developmental Cell 21 (3), S. 445 - 456 (2011)
  37. 37.
    Güttler, T.; Görlich, D.: Ran-dependent nuclear export mediators: A structural perspective. EMBO Journal 30 (17), S. 3457 - 3474 (2011)
  38. 38.
    Madl, T.; Güttler, T.; Görlich, D.; Sattler, M.: Structural analysis of large protein complexes using solvent paramagnetic relaxation enhancements. Angewandte Chemie International Edition 50 (17), S. 3993 - 3997 (2011)
  39. 39.
    Güttler, T.; Madl, T.; Neumann, P.; Deichsel, D.; Lorenzo, C.; Monecke, T.; Ficner, R.; Sattler, M.; Görlich, D.: NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Nature Structural and Molecular Biology 17 (11), S. 1367 - 1376 (2010)
  40. 40.
    Krull, S.; Dörries, J.; Boysen, B.; Reidenbach, S.; Magnius, L.; Norder, H.; Thyberg, J.; Cordes, V. C.: Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion. EMBO Journal 29 (10), S. 1659 - 1673 (2010)
  41. 41.
    Eisele, N. B.; Frey, S.; Piehler, J.; Görlich, D.; Richter, R. P.: Ultrathin nucleoporin FG repeat films and their interaction with nuclear transport receptors. EMBO Reports 11 (5), S. 366 - 372 (2010)
  42. 42.
    Ader, C.; Frey, S.; Maas, W.; Schmidt, H. B.; Görlich, D.; Baldus, M.: Amyloid-like interactions within nucleoporin FG hydrogels. Proceedings of the National Academy of Sciences of the United States of America 107 (14), S. 6281 - 6285 (2010)
  43. 43.
    Chachami, G.; Paraskeva, E.; Mingot, J. M.; Braliou, G. G.; Görlich, D.; Simons, G.: Transport of hypoxia-inducible factor HIF-1α into the nucleus involves importins 4 and 7. Biochemical and Biophysical Research Communications 390 (2), S. 235 - 240 (2009)
  44. 44.
    Mohr, D.; Frey, S.; Fischer, T.; Güttler, T.; Görlich, D.: Characterisation of the passive permeability barrier of nuclear pore complexes. EMBO Journal 28, S. 2541 - 2553 (2009)
  45. 45.
    Frey, S.; Görlich, D.: FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties. EMBO Journal 28, S. 2554 - 2567 (2009)
  46. 46.
    Monecke, T.; Güttler, T.; Neumann, P.; Dickmanns, A.; Görlich, D.; Ficner, R.: Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP. Science 324 (5930), S. 1087 - 1091 (2009)
  47. 47.
    Gontan, C.; Güttler, T.; Engelen, E.; Demmers, J.; Fornerod, M.; Grosveld, F.; Tibboel, D.; Görlich, D.; Poot, A.; Rottier, R.: Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors. Journal of Cell Biology 185 (1), S. 27 - 34 (2009)
  48. 48.
    Weidenfeld, I.; Gossen, M.; Löw, R.; Kentner, D.; Berger, S.; Görlich, D.; Bartsch, D.; Bujard, H.; Schönig, K.: Inducible expression of coding and inhibitory RNAs from retargetable genomic loci. Nucleic Acids Research 37 (7), e50 (2009)
  49. 49.
    Frey, S.; Görlich, D.: A saturated FG-repeat hydrogel can reproduce the permeability properties of nuclear pore complexes. Cell 130 (3), S. 512 - 523 (2007)
  50. 50.
    Frey, S.; Richter, R. P.; Görlich, D.: FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties. Science 314 (5800), S. 815 - 817 (2006)
  51. 51.
    Stavru, F.; Hülsmann, B. B.; Spang, A.; Hartmann, E.; Cordes, V. C.; Görlich, D.: NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes. Journal of Cell Biology 173 (4), S. 509 - 519 (2006)
  52. 52.
    Stavru, F.; Nautrup-Pedersen, G.; Cordes, V. C.; Görlich, D.: Nuclear pore complex assembly and maintenance in POM121-and gp210-deficient cells. Journal of Cell Biology 173 (4), S. 477 - 483 (2006)
  53. 53.
    Bohnsack, M. T.; Stüven, T.; Kuhn, C.; Cordes, V. C.; Görlich, D.: A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes. Nature Cell Biology 8 (3), S. 257 - 263 (2006)
  54. 54.
    Ribbeck, K.; Groen, A. C.; Santarella, R.; Bohnsack, M. T.; Raemaekers, T.; Köcher, T.; Gentzel, M.; Görlich, D.; Wilm, M.; Carmeliet, G. et al.; Mitchison, T. J.; Ellenberg, J.; Hoenger, A.; Mattaj, I. W.: NuSAP, a mitotic RanGTP target that stabilizes and crosslinks microtubules. Molecular Biology of the Cell 17, S. 2646 - 2660 (2006)
  55. 55.
    Hantschel, O.; Wiesner, S.; Güttler, T.; Mackereth, C. D.; Remsing Rix, L. L.; Mikes, Z.; Dehne, J.; Görlich, D.; Sattler, M.; Superti-Furga, G.: Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl. Molecular Cell 19 (4), S. 461 - 473 (2005)
  56. 56.
    Fassati, A.; Görlich, D.; Harrison, I.; Zaytseva, I.; Mingot, J. M.: Nuclear import of HIV‐1 intracellular reverse transcription complexes is mediated by importin 7. The EMBO Journal 22 (14), S. 3675 - 3685 (2003)
  57. 57.
    Bohnsack, M. T.; Regener, K.; Schwappach, B.; Saffrich, R.; Paraskeva, E.; Hartmann, E.; Görlich, D.: Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. The EMBO Journal 21 (22), S. 6205 - 6215 (2002)
  58. 58.
    Köhler, M.; Görlich, D.; Hartmann, E.; Franke, J.: Adenoviral E1A protein nuclear import is preferentially mediated by importin α3 in vitro. Virology 289 (2), S. 186 - 191 (2001)
  59. 59.
    Dean, K. A.; von Ahsen, O.; Görlich, D.; Fried, H. M.: Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. Journal of Cell Science 114 (19), S. 3479 - 3485 (2001)
  60. 60.
    Ferrando-May, E.; Cordes, V.; Biller-Ckovric, I.; Mirkovic, J.; Görlich, D.; Nicotera, P.: Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis. Cell Death and Differentiation 8 (5), S. 495 - 505 (2001)
  61. 61.
    Kutay, U.; Hartmann, E.; Treichel, N.; Calado, A.; Carmo-Fonseca, M.; Prehn, S.; Kraft, R.; Görlich, D.; Bischoff, F. R.: Identification of Two novel RanGTP-binding proteins belonging to the importin β superfamily. The Journal of Biological Chemistry 275 (51), S. 40163 - 40168 (2000)
  62. 62.
    Lippai, M.; Tirián, L.; Boros, I.; Mihály, I.; Erdélyi, M.; Belecz, I.; Máthé, E.; Pósfai, J.; Nagy, A.; Udvardy, A. et al.; Paraskeva, E.; Görlich, D.; Szabad, J.: The Ketel gene encodes a Drosophila homologue of importin-β. Genetics 156 (4), S. 1889 - 1900 (2000)
  63. 63.
    Villa Braslavsky, C. I.; Nowak, C.; Görlich, D.; Wittinghofer, A.; Kuhlmann, J.: Different structural and kinetic requirements for the interaction of Ran with the Ran-binding domains from RanBP2 and importin-β. Biochemistry 39 (38), S. 11629 - 11639 (2000)
  64. 64.
    Bannister, A. J.; Miska, E. A.; Görlich, D.; Kouzarides, T.: Acetylation of importin-α nuclear import factors by CBP/p300. Current Biology 10 (8), S. 467 - 470 (2000)
  65. 65.
    Bachi, A.; Braun, I. C.; Rodrigues, J. P.; Panté, N.; Ribbeck, K.; von Kobbe, C.; Kutay, U.; Wilm, M.; Görlich, D.; Carmo-Fonseca, M. et al.; Itzaurralde, E.: The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. RNA 6 (1), S. 136 - 158 (2000)
  66. 66.
    Köhler, M.; Speck, C.; Christiansen, M.; Bischoff, F. R.; Prehn, S.; Haller, H.; Görlich, D.; Hartmann, E.: Evidence for distinct substrate specificities of importin α family members in nuclear protein import. Molecular and Cellular Biology 19 (11), S. 7782 - 7791 (1999)
  67. 67.
    Görlich, D.: Transport into and out of the cell nucleus. The EMBO Journal 17 (10), S. 2721 - 2727 (1998)
  68. 68.
    Schlenstedt, G.; Smirnova, E.; Deane, R.; Solsbacher, J.; Kutay, U.; Görlich, D.; Ponstingl, H.; Bischoff, F. R.: Yrb4p, a yeast Ran–GTP‐binding protein involved in import of ribosomal protein L25 into the nucleus. The EMBO Journal 16 (20), S. 6237 - 6249 (1997)
  69. 69.
    Deane, R.; Schäfer, W.; Zimmermann, H. P.; Mueller, L.; Görlich, D.; Prehn, S.; Ponstingl, H.; Bischoff, F. R.: Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1. Molecular and Cellular Biology 17 (9), S. 5087 - 5096 (1997)
  70. 70.
    Lewis, J. D.; Görlich, D.; Mattaj, I. W.: A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing. Nucleic Acids Research 24 (17), S. 3332 - 03336 (1996)
  71. 71.
    Oliver, J.; Jungnickel, B.; Görlich, D.; Rapoport, T. A.; High, S.: The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum. FEBS Letters 362 (2), S. 126 - 130 (1995)
  72. 72.
    Kalies, K. U.; Görlich, D.; Rapoport, T. A.: Binding of ribosomes to the rough endoplasmic reticulum mediated by the Sec61p-complex. The Journal of Cell Biology 126 (4), S. 925 - 934 (1994)
  73. 73.
    High, S.; Martoglio, B.; Görlich, D.; Andersen, S. S.; Ashford, A. J.; Giner, A.; Hartmann, E.; Prehn, S.; Rapoport, T. A.; Dobberstein, B.: Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence. The Journal of Biological Chemistry 268 (35), S. 26745 - 26751 (1993)
  74. 74.
    Hartmann, E.; Görlich, D.; Kostka, S.; Otto, A.; Kraft, R.; Knespel, S.; Bürger, E.; Rapoport, T. A.; Prehn, S.: A tetrameric complex of membrane proteins in the endoplasmic reticulum. European Journal of Biochemistry 214 (2), S. 375 - 381 (1993)
  75. 75.
    High, S.; Andersen, S. S.; Görlich, D.; Hartmann, E.; Prehn, S.; Rapoport, T. A.; Dobberstein, B.: Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion. The Journal of Cell Biology 121 (4), S. 743 - 750 (1993)
  76. 76.
    High, S.; Görlich, D.; Wiedmann, M.; Rapoport, T. A.; Dobberstein, B.: The identification of proteins in the proximity of signal-anchor sequences during their targeting to and insertion into the membrane of the ER. The Journal of Cell Biology 113 (1), S. 35 - 44 (1991)
  77. 77.
    Vogel, F.; Hartmann, E.; Görlich, D.; Rapoport, T. A.: Segregation of the signal sequence receptor protein in the rough endoplasmic reticulum membrane. European Journal of Cell Biology 53 (2), S. 197 - 202 (1990)
  78. 78.
    Wiedmann, M.; Görlich, D.; Hartmann, E.; Kurzchalia, T. V.; Rapoport, T. A.: Photocrosslinking demonstrates proximity of a 34 kDa membrane protein to different portions of preprolactin during translocation through the endoplasmic reticulum. FEBS Letters 257 (2), S. 263 - 268 (1989)

Editorial (1)

  1. 79.
    Görlich, D.; Kriwacki, R. W.: Editorial overview: Functional and mechanistic landscape of the nuclear pore complex. Journal of Molecular Biology 428 (10), S. 1947 - 1948 (2016)
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