Biology
 
Cell organelles: structure, function and dynamics
 
 
 

 

Harnessed with our partly unique microscopy and nanoscopy techniques, we are setting out to explore the relationship between the structure and function of selected cellular organelles, such as the mitochondria. At the same time, we use our advanced techniques to map protein distributions at the highest possible temporal and spatial resolution [6, 11, 26, 27].

The animated graphic shows a surface reconstructed 3D-image of the GFP-tagged mitochondrial matrix of a live budding yeast cell. The cell wall is counterstained with the dye calcofluor white. Please click on the animation to load a movie (5,4 MB).

 
 
[6]
Egner, A., S. Jakobs and S. W. Hell (2002). "Fast 100-nm resolution 3D-microscope reveals structural plasticity of mitochondria in live yeast." Proc. Natl. Acad. Sci. USA 99: 3370-3375.
[11] Jakobs, S., N. Martini, A. C. Schauss, A. Egner, B. Westermann and S. W. Hell (2003). "Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p." J. Cell Sci. 116(10): 2005-2014.
[26] Jakobs, S., A. C. Schauss and S. W. Hell (2003). "Photoconversion of matrix targeted GFP enables analysis of continuity and intermixing of the mitochondrial lumen." FEBS Lett. 554: 194-200.
[27]
Messerschmitt, M., S. Jakobs, F. Vogel, S. Fritz, K. S. Dimmer, W. Neupert and B. Westermann (2003). "The inner membrane protein Mdm33 controls mitochondrial morphology in yeast." J. Cell Biol. 160(4): 553-564.