Shvadchak, Volodymyr, Dr.

Research Group

Jovin

Research Interests

Interaction of alpha-synuclein with membranes

• Determination of the binding preferences of AS to membranes of different compositions.
• Dynamics of AS membrane binding
• Studies of AS conformation/position in model membranes (LUVs).
• Role of membranes in AS aggregation.

Interaction of amyloid-beta with membranes

• Membrane binding preferences
• Aggregation and membrane binding

Development and application of environment-sensitive fluorescent labels for monitoring protein interactions

• Developmennt of two-band ratiometric dyes based on 3-hydroxychromones
• New solvatochromic dyes highly sensitive to environment polarity

Alpha-synuclein membrane binding project description

Publications

2011

Shvadchak, V.V., Yushchenko, D.A., Pievo, R. and Jovin, T.M. (2011) The mode of alpha-synuclein binding to membranes depends on lipid composition and lipid to protein ratio. FEBS Lett (Text)

Shvadchak, V.V., Falomir-Lockhart, L.J., Yushchenko, D.A. and Jovin, T.M. (2011) Specificity and kinetics of alpha-synuclein binding to model membranes determined with fluorescent excited state intramolecular proton transfer (ESIPT) probe. J Biol Chem, 286, 13023-13032 (Text)

Shvadchak, V.V., Falomir-Lockhart, L.J., Yushchenko, D.A. and Jovin, T.M. (2011) Interactions of α-Synuclein with Lipids and Artificial Membranes Monitored by ESIPT Probes, in Lipids and Cellular Membranes in Amyloid Diseases (ed R. Jelinek), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527634323.ch1 (Text)

Postupalenko, V. Y., Shvadchak, V. V., Duportail, G., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2011). Monitoring membrane binding and insertion of peptides by two-color fluorescent label. Biochim Biophys Acta 1808, 424-32 (Text)

Zamotaiev, O. M., Postupalenko, V. Y., Shvadchak, V. V., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2011). Improved Hydration-Sensitive Dual-Fluorescence Labels For Monitoring Peptide-Nucleic Acid Interactions. Bioconjug Chem. , 22, 101-107

2010

Choulier, L., Shvadchak, V., Naidoo, A., Klymchenko, A., Mely, Y., Altschuh, D. (2010) A peptide-based fluorescent ratiometric sensor for quantitative detection of proteins. Anal. Biochem., 401, 188-195

2009

Shvadchak, V.V., Klymchenko, A.S., de Rocquigny, H. and Mely, Y. (2009) Sensing peptide–oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein. Nucleic Acids Res , 37, (3), e25.

Shvadchak, V.; Sanglier, S.; Rocle, S.; Villa, P.; Haiech, J.; Hibert, M.; Van Dorsselaer, A.; Mely, Y.; de Rocquigny, H. (2009) Identification by high throughput screening of small compounds inhibiting the nucleic acid destabilization activity of the HIV-1 nucleocapsid protein. Biochimie, 91, (7), 916-923.

Bilokin, M.D., Shvadchak, V.V., Yushchenko, D.A., Duportail, G., Mely, Y. and Pivovarenko, V.G. (2009) Dual-Fluorescence Probe of Environment Basicity (Hydrogen Bond Accepting Ability) Displaying no Sensitivity to Polarity. J Fluoresc., 19, (3), 545-553.

Bilokin, M.D., Shvadchak, V.V., Yushchenko, D.A., Duportail, G., Klymchenko, A.S., Mely, Y. and Pivovarenko, V.G. (2009) 3-Hydroxybenzo[g]quinolones: dyes with red-shifted absorption and highly resolved dual emission. Tetrah Lett, 50(30), 4714-4719.

Spadafora, M., Postupalenko, V.Y., Shvadchak, V.V., Klymchenko, A.S., Mely, Y., Burger, A., and Benhida, R. (2009) Efficient synthesis of ratiometric fluorescent nucleosides featuring 3 hydroxychromone nucleobases. Tetrahedron, 67, (37), 7809-7816.

2008

Klymchenko, A.S., Shvadchak, V.V., Yushchenko, D.A., Jain, N. and Mely, Y. (2008) Excited-state intramolecular proton transfer distinguishes microenvironments in single- and double-stranded DNA. J Phys Chem B, 112, 12050-12055.

de Rocquigny, H., Shvadchak, V., Avilov, S., Dong, C.Z., Dietrich, U., Darlix, J.L. and Mely, Y. (2008) Targeting the viral nucleocapsid protein in anti-HIV-1 therapy. Mini Rev Med Chem, 8, 24-35.

Avilov, S.V., Piemont, E., Shvadchak, V., de Rocquigny, H. and Mely, Y. (2008) Probing dynamics of HIV-1 nucleocapsid protein/target hexanucleotide complexes by 2-aminopurine. Nucleic Acids Res, 36, 885-896.

Pipko, S., Bezgubenko, L., Sinitsa, A., Rusanov, E., Kapustin, E., Povolotskii, M. and Shvadchak, V. (2008) Synthesis and structure of complexes of phosphorus pentachloride with 4-dimethylaminopyridine and N-methytimidazole. Heteroatom Chem, 19, 171-177.

2007

Yushchenko, D.A., Shvadchak, V.V., Klymchenko, A.S., Duportail, G., Pivovarenko, V.G. and Mely, Y. (2007) Modulation of excited-state intramolecular proton transfer by viscosity in protic media. J Phys Chem A, 111, 10435-10438.

Yushchenko, D.A., Shvadchak, V.V., Klymchenko, A.S., Duportail, G., Pivovarenko, V.G. and Mely, Y. (2007) Steric control of the excited-state intramolecular proton transfer in 3-hydroxyquinolones: steady-state and time-resolved fluorescence study. J Phys Chem A, 111, 8986-8992.

M'Baye, G., Klymchenko, A.S., Yushchenko, D.A., Shvadchak, V.V., Ozturk, T., Mely, Y. and Duportail, G. (2007) Fluorescent dyes undergoing intramolecular proton transfer with improved sensitivity to surface charge in lipid bilayers. Photochem Photobiol Sci, 6, 71-76.

2006

Yushchenko, D.A., Shvadchak, V.V., Bilokin, M.D., Klymchenko, A.S., Duportail, G., Mely, Y. and Pivovarenko, V.G. (2006) Modulation of dual fluorescence in a 3-hydroxyquinolone dye by perturbation of its intramolecular proton transfer with solvent polarity and basicity. Photochem Photobiol Sci, 5, 1038-1044.

Yushchenko, D., Shvadchak, V., Klymchenko, A., Duportail, G., Mely, Y. and Pivovarenko, V. (2006) 2 Aryl-3-hydroxyquinolones, a new class of dyes with solvent dependent dual emission due to excited state intramolecular proton transfer. New J Chem, 30, 774-781.

Pipko, S., Simurova, N., Shvadchak, V., Bezgubenko, L. and Luk'yanenko, S. (2006) Dichlorophosphate anion in the synthesis of thioamides. Russ J Gen Chem+, 76, 1019-1021.

Phone

+49-551-201-1392

Email

volodymyr.shvadchak{at}mpibpc.mpg.de