• reconstructs nanometer distance trajectories from time-resolved
  single photon FRET recordings,
• extracts the full information from the recorded photon arrival times,
• provides rigorous error bounds and 
• is able to extract an effective diffusion coefficient for the distance fluctuations.

 _      _     FRETtrace 1.0   (C) 2002 Helmut Grubmüller, Gunnar Schröder
  \    / \   _     Purpose: Create maximum likelihood trajectories
   \  /   \_/               from single molecule FRET data
    \/          www.mpibpc.mpg.de/groups/grubmueller/start/people/hgrub ; hgrubmu@gwdg.de
               Reference: G. Schroder and H. Grubmuller (submitted)
 

  Reading photon arrival times from file burst.dat ... Done.
    Read 230 photons (117 donor, 113 acceptor).
    Start time: 7545472.000000 mu s    End time: 7555268.500000 mu s   (Length: 9796.500000 mu s)
  Output file for distance distribution map or likelihood: burst.out
  Foerster radius: 6.5 nm
  Smallest distance considered 0 nm (internal: -13 nm)
  Largest  distance considered 13 nm (internal: 26 nm)
  Number of grid points for time axis: 1000
  Number of grid points for distance axis: 400 (internal: 1200)
  Will calculate distance distribution for diffusion coefficient D = 1e-14 m^2/s
 

Forward scan photons ...  100%  Done.
Backward scan photons ... 100%  Done.
Forward scan grid ...     100%  Done.
Backward scan grid ...    100%  Done.

Writing distance distribution P(r,t) to file burst.out ... Done.
 

FRETtrace ready. No errors.