Journal Article (97)

  1. Journal Article
    Kastner, B.; Will, C. L.; Stark, H.; Lührmann, R.: Structural insights into nuclear pre-mRNA splicing in higher eukaryotes. Cold Spring Harbor Perspectives in Biology, (in press) (2019)
  2. Journal Article
    Watson, E. R.; Brown, N. G.; Peters, J. M.; Stark, H.; Schulman, B. A.: Posing the APC/C E3 ubiquitin ligase to orchestrate cell division. Trends in Cell Biology 29 (2), pp. 117 - 134 (2019)
  3. Journal Article
    Haselbach, D.; Komarov, I.; Agafonov, D. E.; Hartmuth, K.; Graf, B.; Dybkov, O.; Urlaub, H.; Kastner, B.; Lührmann, R.; Stark, H.: Structure and conformational dynamics of the human spliceosomal Bact complex. Cell 172 (3), pp. 454 - 464, e1-e5 (2018)
  4. Journal Article
    Renaud, J. P.; Chari, A.; Ciferri, C.; Liu, W.; Rémigy, H. W.; Stark, H.; Wiesmann, C.: Cryo-EM in drug discovery: Achievements, limitations and prospects. Nature Reviews Drug Discovery 17, pp. 471 - 492 (2018)
  5. Journal Article
    Bertram, K.; Agafonov, D. E.; Dybkov, O.; Haselbach, D.; Leelaram, M. N.; Will, C. L.; Urlaub, H.; Kastner, B.; Lührmann, R.; Stark, H.: Cryo-EM structure of a pre-catalytic human spliceosome primed for activation. Cell 170 (4), pp. 701 - 713, e1 - e4 (2017)
  6. Journal Article
    Haselbach, D.; Schrader, J.; Lambrecht, F.; Henneberg, F.; Chari, A.; Stark, H.: Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs. Nature Communications 8, 15578 (2017)
  7. Journal Article
    Rodnina, M. V.; Fischer, N.; Maracci, C.; Stark, H.: Ribosome dynamics during decoding. Philosophical Transactions of the Royal Society B 372 (1716), 20160182 (2017)
  8. Journal Article
    Bertram, K.; Agafonov, D. E.; Liu, W.; Dybkov, O.; Will, C. L.; Hartmuth, K.; Urlaub, H.; Kastner, B.; Stark, H.; Lührmann, R.: Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature 542 (7641), pp. 318 - 323 (2017)
  9. Journal Article
    Fischer, N.; Neumann, P.; Bock, l. V.; Maracci, C.; Wang, Z.; Paleskava, A.; Konevega, A. L.; Schröder, G. F.; Grubmüller, H.; Ficner, R. et al.; Rodnina, M. V.; Stark, H.: The pathway to GTPase activation of elongation factor SelB on the ribosome. Nature 540 (7631), pp. 80 - 85 (2016)
  10. Journal Article
    Rauhut, R.; Fabrizio, P.; Dybkov, O.; Hartmuth, K.; Pena, V.; Chari, A.; Kumar, V.; Lee, C. T.; Urlaub, H.; Kastner, B. et al.; Stark, H.; Lührmann, R.: Molecular architecture of the Saccharomyces cerevisiae activated spliceosome. Science 353 (6306), pp. 1399 - 1405 (2016)
  11. Journal Article
    Paknia, E.; Chari, A.; Stark, H.; Fischer, U.: The ribosome cooperates with the assembly chaperone pICln to initiate formation of snRNPs. Cell Reports 16 (12), pp. 3103 - 3112 (2016)
  12. Journal Article
    Agafonov, D. E.; van Santen, M.; Kastner, B.; Dube, P.; Will, C. L.; Urlaub, H.; Lührmann, R.: ATPγS stalls splicing after B complex formation but prior to spliceosome activation. RNA 22 (9), pp. 1329 - 1337 (2016)
  13. Journal Article
    Hons, M.; Huis in ‘t Veld, P. J.; Kaesler, J.; Rombaut, P.; Schleiffer, A.; Herzog, F.; Stark, H.; Peters, J. M.: Topology and structure of an engineered human cohesin complex bound to Pds5B. Nature Communications 7, 12523 (2016)
  14. Journal Article
    Yamaguchi, M.; VanderLinden, R.; Weissmann, F.; Qiao, R.; Dube, P.; Brown, N. G.; Haselbach, D.; Zhang, W.; Sidhu, S. S.; Peters, J. M. et al.; Stark, H.; Schulman, B. A.: Cryo-EM of mitotic checkpoint complex-bound APC/C reveals reciprocal and conformational regulation of ubiquitin ligation. Molecular Cell 63 (4), pp. 593 - 607 (2016)
  15. Journal Article
    Schrader, J.; Henneberg, F.; Mata, R. A.; Tittmann, K.; Schneider, T. R.; Stark, H.; Bourenkov, G.; Chari, A.: The inhibition mechanism of human 20S proteasomes enables next-generation inhibitor design. Science 353 (6299), pp. 594 - 598 (2016)
  16. Journal Article
    Brown, N. G.; VanderLinden, R.; Watson, E. R.; Weissmann, F.; Ordureau, A.; Wu, K. P.; Zhang, W.; Yu, S.; Mercredi, P. Y.; Harrison, J. S. et al.; Davidson, I. F.; Qiao, R. P.; Lu, Y.; Dube, P.; Brunner, M. R.; Grace, C. R. R.; Miller, D. J.; Haselbach, D.; Jarvis, M. A.; Yamaguchi, M.; Yanishevski, D.; Petzold, G.; Sidhu, S. S.; Kuhlman, B.; Kirschner, M. W.; Harper, J. W.; Peters, J. M.; Stark, H.; Schulman, B. A.: Dual RING E3 architectures regulate multiubiquitination and ubiquitin chain elongation by APC/C. Cell 165 (6), pp. 1440 - 1453 (2016)
  17. Journal Article
    Qiao, R. P.; Weissmann, F.; Yamaguchi, M.; Brown, N. G.; VanderLinden, R.; Imre, R.; Jarvis, M. A.; Brunner, M. R.; Davidson, I. F.; Litos, G. et al.; Haselbach, D.; Mechtler, K.; Stark, H.; Schulman, B. A.; Peters, J. M.: Mechanism of APC/C-CDC20 activation by mitotic phosphorylation. Proceedings of the National Academy of Sciences of the United States of America 113 (19), pp. E2570 - E2578 (2016)
  18. Journal Article
    Weissmann, F.; Petzold, G.; VanderLinden, R.; in'T Veld, P. J. H.; Brown, N. G.; Lampert, F.; Westermann, S.; Stark, H.; Schulman, B. A.; Peters, J. M.: biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Proceedings of the National Academy of Sciences of the United States of America 113 (19), pp. E2564 - E2569 (2016)
  19. Journal Article
    Agafonov, D. E.; Kastner, B.; Dybkov, O.; Hofele, R. V.; Liu, W.; Urlaub, H.; Lührmann, R.; Stark, H.: Molecular architecture of the human U4/U6.U5 tri-snRNP. Science 351 (6280), pp. 1416 - 1420 (2016)
  20. Journal Article
    Stark, H.; Chari, A.: Sample preparation of biological macromolecular assemblies for the determination of high-resolution structures by cryo-electron microscopy. Microscopy 65 (1), pp. 23 - 34 (2016)
  21. Journal Article
    Pleiner, T.; Bates, M.; Trakhanov, S.; Lee, C. T.; Schliep, J. E.; Chug, H.; Böhning, M.; Stark, H.; Urlaub, H.; Görlich, D.: Nanobodies: Site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife 4, e11349 (2015)
  22. Journal Article
    Chari, A.; Haselbach, D.; Kirves, J. M.; Ohmer, J.; Paknia, E.; Fischer, N.; Ganichkin, O.; Moller, V.; Frye, J. J.; Petzold, G. et al.; Jarvis, M.; Tietzel, M.; Grimm, C.; Peters, J. M.; Schulman, B. A.; Tittmann, K.; Markl, J.; Fischer, U.; Stark, H.: ProteoPlex: Stability optimization of macromolecular complexes by sparse-matrix screening of chemical space. Nature Methods 12 (9), pp. 859 - 865 (2015)
  23. Journal Article
    Hauer, F.; Gerle, C.; Fischer, N.; Oshima, A.; Shinzawa-Itoh, K.; Shimada, S.; Yokoyama, K.; Fujiyoshi, Y.; Stark, H.: GraDeR: Membrane protein complex preparation for single-particle cryo-EM. Structure 23 (9), pp. 1769 - 1775 (2015)
  24. Journal Article
    Lin , Y. J.; Liu, W.; Stark, H.; Huang, C. T.: Expression of enterovirus 71 virus-like particles in transgenic enoki (Flammulina velutipes). Applied Microbiology and Biotechnology 99 (16), pp. 6765 - 6774 (2015)
  25. Journal Article
    Martinez-Rucobo, F. W.; Kohler, R.; Waterbeemd, M. v. d.; Heck, A. J.; Hemann, M.; Herzog, F.; Stark, H.; Cramer, P.: Molecular basis of transcription-coupled pre-mRNA capping. Molecular Cell 58 (6), pp. 1079 - 1089 (2015)
  26. Journal Article
    Neuenkirchen, N.; Englbrecht, C.; Ohmer, J.; Ziegenhals, T.; Chari, A.; Fischer, U.: Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization. EMBO Journal 34 (14), pp. 1925 - 1941 (2015)
  27. Journal Article
    Brown, N. G.; VanderLinden, R.; Watson, E. R.; Qiao, R. P.; Grace, C. R. R.; Yamaguchi, M.; Weissmann, F.; Frye, J. J.; Dube, P.; Cho, S. E. et al.; Actis, M. L.; Rodrigues, P.; Fujii, N.; Peters, J. M.; Stark, H.; Schulman, B. A.: RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex. Proceedings of the National Academy of Sciences of the United States of America 112 (17), pp. 5272 - 5279 (2015)
  28. Journal Article
    Fischer, N.; Neumann, P.; Konevega, A. L.; Bock, L. V.; Ficner, R.; Rodnina, M. V.; Stark, H.: Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM. Nature 520 (7548), pp. 567 - 570 (2015)
  29. Journal Article
    Brown, N. G.; Watson, E. R.; Weissmann, F.; Jarvis, M. A.; VanderLinden, R.; Grace, C.R.R.; Frye, J. J.; Qiao, R.; Dube, P.; Petzold, G. et al.; Cho, S. E.; Alsharif, O.; Bao, J.; Davidson, I.F.; Zheng, J. J.; Nourse, A.; Kurinov, I.; Peters, J. M.; Stark, H.; Schulman, B. A.: Mechanism of polyubiquitination by human anaphase-promoting complex: RING repurposing for ubiquitin chain assembly. Molecular Cell 56 (2), pp. 246 - 260 (2014)
  30. Journal Article
    Liu, Y.; Neumann, P.; Kuhle, B.; Monecke, T.; Schell, S.; Chari, A.; Ficner, R.: Translation initiation factor eIF3b contains a nine-bladed beta-propeller and interacts with the 40S ribosomal subunit. Structure 22 (6), pp. 923 - 930 (2014)
  31. Journal Article
    Bock, L. V.; Blau, C.; Schröder, G. F.; Davydov, I. I.; Fischer, N.; Stark, H.; Rodnina, M. V.; Vaiana, A. C.; Grubmüller, H.: Energy barriers and driving forces in tRNA translocation through the ribosome. Nature Structural and Molecular Biology 20 (12), pp. 1390 - 1396 (2013)
  32. Journal Article
    Doelker, N.; Blanchet, C. E.; Voss, B.; Haselbach, D.; Kappel, C.; Monecke, T.; Svergun, D. I.; Stark, H.; Ficner, R.; Zachariae, U. et al.; Grubmüller, H.; Dickmanns, A.: Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21 (8), pp. 1350 - 1360 (2013)
  33. Journal Article
    Frye, J.; Brown, N. G.; Petzold, G.; Watson, E. R.; Grace, C. R. R.; Nourse, A.; Jarvis, M. A.; Kriwacki, R. W.; Peters, J. M.; Stark, H. et al.; Schulman, B. A.: Electron microscopy structure of human APC/C-CDH1-EMI1 reveals multimodal mechanism of E3 ligase shutdown. Nature Structural and Molecular Biology 20 (7), pp. 827 - 835 (2013)
  34. Journal Article
    Hauer, F.; Gerle, C.; Kirves, J. M.; Stark, H.: Automated correlation of single particle tilt pairs for Random Conical Tilt and Orthogonal Tilt Reconstructions. Journal of Structural Biology 181 (2), pp. 149 - 154 (2013)
  35. Journal Article
    Grimm, C.; Chari, A.; Pelz, J. P.; Kuper, J.; Kisker, C.; Diederichs, K.; Stark, H.; Schindelin, H.; Fischer, U.: Structural basis of assembly chaperone- mediated snRNP formation. Molecular Cell 49 (4), pp. 692 - 703 (2013)
  36. Journal Article
    Monecke, T.; Haselbach, D.; Voß, D.; Russek, A.; Neumann, P.; Thomson, E.; Hurt, E.; Zachariae, U.; Stark, H.; Grubmüller, H. et al.; Dickmanns, A.; Ficner, R.: Structural basis for cooperativity of CRM1 export complex formation. Proceedings of the National Academy of Sciences of the United States of America 110 (3), pp. 960 - 965 (2013)
  37. Journal Article
    Khoshnevis, S.; Hauer, F.; Milon, P.; Stark, H.; Ficner, R.: Novel insights into the architecture and protein interaction network of yeast eIF3. RNA 18 (12), pp. 2306 - 2319 (2012)
  38. Journal Article
    Uzunova, K.; Dye, B. T.; Schutz, H.; Ladurner, R.; Petzold, G.; Toyoda, Y.; Jarvis, M. A.; Brown, N. G.; Poser, I.; Novatchkova, M. et al.; Mechtler, K.; Hyman, A. A.; Stark, H.; Schulman, B. A.; Peters, J. M.: APC15 mediates CDC20 autoubiquitylation by APC/C-MCC and disassembly of the mitotic checkpoint complex. Nature Structural and Molecular Biology 19 (11), pp. 1116 - 1123 (2012)
  39. Journal Article
    Brunotte, L.; Kerber, R.; Shang, W.; Hauer, F.; Hass, M.; Gabriel, M.; Lelke, M.; Busch, C.; Stark, H.; Svergun, D. I. et al.; Betzel, C.; Perbrandt, M.; Guenther, S.: Structure of the Lassa virus nucleoprotein revealed by X-ray crystallography, small-angle X-ray scattering, and electron microscopy. Journal of Biological Chemistry 286 (44), pp. 38748 - 38756 (2011)
  40. Journal Article
    Nikolov, M.; Stützer, A.; Mosch, K.; Krasauskas, A.; Soeroes, S.; Stark, H.; Urlaub, H.; Fischle, W.: Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns. Molecular and Cellular Proteomics 10 (11), pp. 10.1074/mcp.M110.005371-1 - 10.1074/mcp.M110.005371–16 (2011)
  41. Journal Article
    Buschhorn, B. A.; Petzold, G.; Galova, M.; Dube, P.; Kraft, C.; Herzog, F.; Stark, H.; Peters, J. M.: Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nature Structural and Molecular Biology 18, pp. 6 - 13 (2010)
  42. Journal Article
    Golas, M. M.; Sander, B.; Bessonov, S.; Grote, M.; Wolf, E.; Kastner, B.; Stark, H.; Lührmann, R.: 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core. Molecular Cell 40 (6), pp. 927 - 938 (2010)
  43. Journal Article
    Bessonov, S.; Anokhina, M.; Krasauskas, A.; Golas, M. M.; Sander, B.; Will, C. L.; Urlaub, H.; Stark, H.; Lührmann, R.: Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis. RNA 16 (12), pp. 2384 - 2403 (2010)
  44. Journal Article
    Petrovic, A.; Pasqualato, S.; Dube, P.; Krenn, V.; Santaguida, S.; Cittaro, D.; Monzani, S.; Massimiliano, L.; Keller, J.; Tarricone, A. et al.; Maiolica, A.; Stark, H.; Musaccio, A.: The MIS12 complex is a protein interaction hub for outer kinetochore assembly. Journal of Cell Biology 190 (5), pp. 835 - 852 (2010)
  45. Journal Article
    Sander, B.; Golas, M. M.; Lührmann, R.; Stark, H.: An approach for de novo structure determination of dynamic molecular assemblies by electron cryomicroscopy. Structure 18 (6), pp. 667 - 676 (2010)
  46. Journal Article
    Hutchins, J. R. A.; Toyoda, Y.; Hegemann, B.; Poser, I.; Heriche, J. K.; Sykora, M. M.; Augsburg, M.; Hudecz, O.; Buschhorn, B. A.; Bulkescher, J. et al.; Conrad, C.; Comartin, D.; Schleiffer, A.; Sarov, M.; Pozniakovsky, A.; Slabicki, M. M.; Schloissnig, S.; Steinmacher, I.; Leuschner, M.; Ssykor, A.; Lawo, S.; Pelletier, L.; Stark, H.; Nasmyth, K.; Ellenberg, J.; Durbin, R.; Buchholz, F.; Mechtler, K.; Hyman, A. A.; Peters, J. M.: Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science 328 (5978), pp. 593 - 599 (2010)
  47. Journal Article
    Fischer, N.; Konevega, A. L.; Wintermeyer, W.; Rodnina, M. V.; Stark, H.: Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy. Nature 466 (7304), pp. 329 - 333 (2010)
  48. Journal Article
    Richter, F. M.; Sander, B.; Golas, M. M.; Stark, H.; Urlaub, H.: Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion. Molecular and Cellular Proteomics 9, pp. 1729 - 1741 (2010)
  49. Journal Article
    Uezguen, S.; Akdemir, O.; Hasenpusch, G.; Mauksch, C.; Golas, M. M.; Sander, B.; Stark, H.; Imker, R.; Lutz, J. F.; Rudolph, C.: Characterization of tailor-made copolymers of oligo(ethylene glycol) methyl ether methacrylate and N,N-dimethylaminoethyl methacrylate as nonviral gene transfer agents: Influence of macromolecular structure on gene vector particle properties and transfection efficiency. Biomacromolecules 11 (1), pp. 39 - 50 (2010)
  50. Journal Article
    Warkocki, Z.; Odenwälder, P.; Schmitzova, J.; Platzmann, F.; Stark, H.; Urlaub, H.; Ficner, R.; Fabrizio, P.; Lührmann, R.: Reconstitution of both steps of S. cerevisiae splicing with purified spliceosomal components. Nature Structural and Molecular Biology 16 (12), pp. 1237 - 1243 (2009)
  51. Journal Article
    Elfinger, M.; Pfeifer, C.; Uezguen, S.; Golas, M. M.; Sander, B.; Mauksch, C.; Stark, H.; Aneja, M. K.; Rudolph, C.: Self-assembly of ternary insulin−polyethylenimine (PEI)−DNA nanoparticles for enhanced gene delivery and expression in alveolar epithelial cells. Biomacromolecules 10 (10), pp. 2912 - 2920 (2009)
  52. Journal Article
    Wolf, E.; Kastner, B.; Deckert, J.; Merz, C.; Stark, H.; Lührmann, R.: Exon, intron and splice site locations in the spliceosomal B complex. EMBO Journal 28, pp. 2283 - 2292 (2009)
  53. Journal Article
    Andersen, E. S.; Dong, M.; Nielsen, M. M.; Jahn, K.; Subramani, R.; Mamdouh, W.; Golas, M. M.; Sander, B.; Stark, H.; Oliveira, C. L. et al.; Pedersen, J. S.; Birkedal, V.; Besenbacher, F.; Gothelf, K. V.; Kjems, J.: Self-assembly of a nanoscale DNA box with a controllable lid. Nature 459 (7243), pp. 73 - 76 (2009)
  54. Journal Article
    Golas, M. M.; Boehm, C.; Sander, B.; Effenberger, K.; Brecht, M.; Stark, H.; Goeringer, H. U.: Snapshots of the RNA editing machine in trypanosomes captured at different assembly stages in vivo. EMBO Journal 28 (6), pp. 766 - 778 (2009)
  55. Journal Article
    Herzog, F.; Primorac, I.; Dube, P.; Lenart, P.; Sander, B.; Mechtler, K.; Stark, H.; Peters, J. M.: Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science 323 (5920), pp. 1477 - 1481 (2009)
  56. Journal Article
    Fabrizio, P.; Dannenberg, J.; Dube, P.; Kastner, B.; Stark, H.; Urlaub, H.; Lührmann, R.: The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Molecular Cell 36 (4), pp. 593 - 608 (2009)
  57. Journal Article
    Lührmann, R.; Stark, H.: Structural mapping of spliceosomes by electron microscopy. Current Opinion in Structural Biology 19, pp. 96 - 102 (2009)
  58. Journal Article
    Sanchez, M.; Drechsler, M.; Stark, H.; Lipps, G.: DNA translocation activity of the multifunctional replication protein ORF904 from the archaeal plasmid pRN1. Nucleic Acids Research 37 (20), pp. 6831 - 6848 (2009)
  59. Journal Article
    Schmeisser, M.; Heisen, B.; Luettich, M.; Busche, B.; Hauer, F.; Koske, T.; Knauber, K. H.: Parallel, distributed and GPU computing technologies in single-particle electron microscopy. Acta Crystallographica D 65 (7), pp. 659 - 671 (2009)
  60. Journal Article
    Karaduman, R.; Dube, P.; Stark, H.; Fabrizio, P.; Kastner, B.; Luehrmann, R.: Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy. RNA 14, pp. 2528 - 2537 (2008)
  61. Journal Article
    Haecker, I.; Sander, B.; Golas, M. M.; Wolf, E.; Karagoez, E.; Kastner, B.; Stark, H.; Fabrizio, P.; Luehrmann, R.: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nature Structural and Molecular Biology 15 (11), pp. 1206 - 1212 (2008)
  62. Journal Article
    Chari, A.; Golas, M. M.; Klingenhaeger, M.; Neuenkirchen, N.; Sander, B.; Englbrecht, C.; Sickmann, A.; Stark, H.; Fischer, U.: An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell 135 (3), pp. 497 - 509 (2008)
  63. Journal Article
    Kastner, B.; Fischer, N.; Golas, M. M.; Sander, B.; Dube, P.; Boehringer, D.; Hartmuth, K.; Deckert, J.; Hauer, F.; Wolf, E. et al.; Uchtenhagen, H.; Urlaub, H.; Herzog, F.; Peters, J. M.; Porschke, D.; Luehrmann, R.; Stark, H.: GraFix: sample preparation for single-particle electron cryomicroscopy. Nature Methods 5 (1), pp. 53 - 55 (2008)
  64. Journal Article
    Sirajuddin, M.; Farkasovsky, M.; Hauer, F.; Kuehlmann, D.; Macara, I. G.; Weyand, M.; Stark, H.; Wittinghofer, A.: Structural insight into filament formation by mammalian septins. Nature 449 (7160), pp. 311 - 315 (2007)
  65. Journal Article
    Kuehn-Hoelsken, E.; Dybkov, O.; Sander, B.; Luehrmann, R.; Urlaub, H.: Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry. Nucleic Acids Research 35 (15), e95 (2007)
  66. Journal Article
    Behzadnia, N.; Golas, M. M.; Hartmuth, K.; Sander, B.; Kastner, B.; Deckert, J.; Dube, P.; Will, C. L.; Urlaub, H.; Stark, H. et al.; Luehrmann, R.: Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes. The EMBO Journal 26 (6), pp. 1737 - 1748 (2007)
  67. Journal Article
    Sander, B.; Golas, M. M.; Makarov, E. M.; Brahms, H.; Kastner, B.; Luehrmann, R.; Stark, H.: Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy. Molecular Cell 24 (2), pp. 267 - 278 (2006)
  68. Journal Article
    Deckert, J.; Hartmuth, K.; Boehringer, D.; Behzadnia, N.; Will, C. L.; Kastner, B.; Stark, H.; Urlaub, H.; Luehrmann, R.: Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions. Molecular and Cellular Biology 26 (14), pp. 5528 - 5543 (2006)
  69. Journal Article
    Schellenberg, M. J.; Edwards, R. A.; Ritchie, D. B.; Kent, O. A.; Golas, M. M.; Stark, H.; Luehrmann, R.; Glover, J. N. M.; MacMilan, A. M.: Crystal structure of a core spliceosomal protein interface. Proceedings of the National Academy of Sciences of the United States of America 103 (5), pp. 1266 - 1271 (2006)
  70. Journal Article
    Stark, H.; Luehrmann, R.: Cryo-electron microscopy of spliceosomal components. Annual Review of Biophysics and Biomolecular Structure 35, pp. 435 - 457 (2006)
  71. Journal Article
    Kuehn-Hoelsken, E.; Lenz, C.; Sander, B.; Luehrmann, R.; Urlaub, H.: Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles. RNA 11 (12), pp. 1915 - 1930 (2005)
  72. Journal Article
    Golas, M. M.; Sander, B.; Will, C. L.; Luehrmann, R.; Stark, H.: Major conformational change in the complex SF3b upon integration into the spliceosomal U11/U12 di-snRNP as revealed by electron cryomicroscopy. Molecular Cell 17 (6), pp. 869 - 883 (2005)
  73. Journal Article
    Boehringer, D.; Thermann, R.; Ostareck-Lederer, A.; Lewis, J. D.; Stark, H.: Structure of the hepatitis C virus IRES bound to the human 80S ribosome: Remodeling of the HCV IRES. Structure 13, pp. 1695 - 1706 (2005)
  74. Journal Article
    Diaconu, M.; Kothe, U.; Schluenzen, F.; Fischer, N.; Harms, J.; Tonevitsky, A. G.; Stark, H.; Rodnina, M. V.; Wahl, M. C.: Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation. Cell 121, pp. 991 - 1004 (2005)
  75. Journal Article
    Dube, P.; Herzog, F.; Gieffers, C.; Sander, B.; Riedel, D.; Mueller, S.; Engel, A.; Peters, J. M.; Stark, H.: Localization of the Coactivator Cdh1 and the Cullin Subunit Apc2 in a Cryo-Electron Microscopy Model of Vertebrate APC/C. Molecular Cell 20, pp. 867 - 879 (2005)
  76. Journal Article
    Rudolph, C.; Schillinger, U.; Ortiz, A.; Plank, C.; Golas, M. M.; Sander, B.; Stark, H.; Rosenecker, J.: Aerosolized nanogram quantities of plasmid DNA mediate highly efficient gene delivery to mouse airway epithelium. Molecular Therapy 12, pp. 493 - 501 (2005)
  77. Journal Article
    Sander, B.; Golas, M. M.; Stark, H.: Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy. Journal of Structural Biology 151, pp. 92 - 105 (2005)
  78. Journal Article
    Tichelaar, W.; Safferling, M.; Keinanen, K.; Stark, H.; Madden, D. R.: The three-dimensional structure of an ionotropic glutamate receptor reveals a dimer-of-dimers assembly. Journal of Molecular Biology 344 (2), pp. 435 - 442 (2004)
  79. Journal Article
    Boehringer, D.; Makarov, E. M.; Sander, B.; Makarova, O.; Kastner, B.; Luehrmann, R.; Stark, H.: Three-dimensional structure of a pre-catalytic human spliceosomal complex B. Nature Structural and Molecular Biology 11 (5), pp. 463 - 468 (2004)
  80. Journal Article
    Stark, H.: Elektronenmikroskopische 3D-Strukturbestimmung makromolekularer Komplexe. BIOspektrum 5, pp. 636 - 638 (2004)
  81. Journal Article
    Sander, B.; Golas, M. M.; Stark, H.: Corrim-based alignment for improved speed in single-particle image processing. Journal of Structural Biology 143 (3), pp. 219 - 228 (2003)
  82. Journal Article
    Sander, B.; Golas, M. M.; Stark, H.: Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra. Journal of Structural Biology 142 (3), pp. 392 - 401 (2003)
  83. Journal Article
    Meissner, U.; Stohr, M.; Kusche, K.; Burmester, T.; Stark, H.; Harris, J. R.; Orlova, E. V.; Markl, J.: Quaternary structure of the European spiny lobster (Palinurus elephas) 1 x 6-mer hemocyanin from cryoEM and amino acid sequence data. Journal of Molecular Biology 325 (1), pp. 99 - 109 (2003)
  84. Journal Article
    Golas, M. M.; Sander, B.; Will, C. L.; Luehrmann, R.; Stark, H.: Molecular architecture of the multiprotein splicing factor SF3b. Science 300, pp. 980 - 984 (2003)
  85. Journal Article
    Stark, H.; Rodnina, M. V.; Wieden, H. J.; Zemlin, F.; Wintermeyer, W.; van Heel, M.: Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex. Nature Structural Biology 9 (11), pp. 849 - 854 (2002)
  86. Journal Article
    Gieffers, C.; Dube, P.; Harris, J. R.; Stark, H.; Peters, J. M.: Three-dimensional structure of the anaphase-promoting complex. Molecular Cell 7, pp. 907 - 913 (2001)
  87. Journal Article
    Maurer, N.; Wong, K. F.; Stark, H.; Louie, L.; McIntosh, D.; Wong, T.; Scherrer, P.; Semple, S. C.; Cullis, P. R.: Spontaneous Entrapment of Polynucleotides upon Electrostatic Interaction with Ethanol-Destabilized Cationic Liposomes. Biophysical Journal 80, pp. 2310 - 2326 (2001)
  88. Journal Article
    Semple, S. C.; Klimuk, S. K.; Harasym, T. O.; Dos Santos, N.; Ansell, S. M.; Wong, K. F.; Maurer, N.; Stark, H.; Cullis, P. R.; Hope, M. J. et al.; Scherrer, P.: Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures. Biochimica et Biophysica Acta 1510, pp. 152 - 166 (2001)
  89. Journal Article
    Stark, H.; Dube, P.; Luehrmann, R.; Kastner, B.: Arrangement of RNA and Proteins in the spliceosomal U1 snRNP. Nature 409, pp. 539 - 542 (2001)
  90. Journal Article
    Stark, H.; Dube, P.; Luehrmann, R.; Kastner, B.: The 3-D arrangement for RNA and Proteins in the spliceosomal U1 snRNP. Nature 409, pp. 539 - 542 (2001)
  91. Journal Article
    Stewart, L.; Manvell, M.; Hilery, E.; Etheridge, C. J.; Cooper, R. G.; Stark, H.; van Heel, M.; Preuss, M.; Alton, E.; Miller, A.: Physico-chemical analysis of cationic liposome-DNA complexes (lipoplexes) with respect to in vitro and in vivo gene delivery efficiency. Journal of Chemical Society 2, pp. 624 - 632 (2001)
  92. Journal Article
    Meissner, U.; Dube, P.; Harris, J. R.; Stark, H.; Markl, J.: Structure of a molluscan hemocyanin didecamer (HtH 1 from Haliotis tuberculata) at 12 A resolution by cryoelectron microscopy. Journal of Molecular Biology 298, pp. 21 - 34 (2000)
  93. Journal Article
    Tam, P.; Monck, M.; Lee, D.; Ludkovski, O.; Leng, E. C.; Clow, K.; Stark, H.; Scherrer, P.; Graham, R. W.; Cullis, P. R.: Stabilized plasmid-lipid particles for systemic gene therapy. Gene Therapy 7, pp. 1867 - 1874 (2000)
  94. Journal Article
    van Heel, M.; Gowen, B.; Matadeen, R.; Orlova, E. V.; Finn, R.; Pape, T.; Cohen, D.; Stark, H.; Schmidt, R.; Schatz, M. et al.; Patwardhan, A.: Single-particle electron cryo-micrsocopy: towards atomic resolution. Quarterly Reviews of Biophysics 33, pp. 307 - 369 (2000)
  95. Journal Article
    Harding, L. B.; Stark, H.; Troe, J.; Ushakov, V. G.: New studies of the unimolecular reaction NO2  O + No. II. Relation between high pressure rate constants and potential parameters. Physical Chemistry Chemical Physics 1, pp. 63 - 72 (1999)
  96. Journal Article
    Harms, J.; Tocilj, A.; Levin, I.; Agmon, I.; Stark, H.; Kolln, I.; van Heel, M.; Cuff, M.; Schluenzen, F.; Bashan, A. et al.; Franceschi, F.; Yonath, A.: Elucidating the medium-resolution structure of ribosomal particles: an interplay between electron cryo-microscopy and x-ray crystallography. Structure Folding and Design 7, pp. 931 - 941 (1999)
  97. Journal Article
    Hippler, H.; Siefke, M.; Stark, H.; Troe, J.: New studies of the unimolecular reaction NO2  O + No. I. High pressure range of the O + NO recombination between 200 and 400 K. Physical Chemistry - Chemical Physics 1, pp. 57 - 61 (1999)

Book Chapter (4)

  1. Book Chapter
    Göringer, H. U.; Stark, H.; Böhm, C.; Sander, B.; Golas, M. M.: Three-dimensional reconstruction of Trypanosoma brucei editosomes using single-particle electron microscopy. In: RNA and DNA editing: Methods and protocols, pp. 3 - 22 (Ed. Aphasizhev, R.). Humana Pr.; Springer, New York (2011)
  2. Book Chapter
    Wintermeyer, W.; Savelsbergh, A.; Konevega, A. L.; Peske, F.; Katunin, V.; Semenkov, Y.; Fischer, N.; Stark, H.; Rodnina, M.: Functions of elongation factor G in translocation and ribosome recycling. In: Ribosomes: Structure, Function, and Dynamics, pp. 329 - 338. Springer, Wien (2011)
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