FILM Microscopes
Four generations of confocal laser scanning microscopes:
Leica TCS SPE, providing excitation wavelengths of 488, 561 and 635 nm. In addition it has a widefield UV illumination. This system comes with only one PMT detector. Multicolor imaging is thus only possible in sequential mode using the ultra high dynamic PMT.
Leica TCS SP5, providing excitation wavelengths of 458, 476, 488, 496, 514, 561 and 633 nm. In addition it has an UV diode laser with excitation at 405 nm. Besides the detection system for 4 fluorescence channels simultaneously and one transmission channel, this systems is also equipped with an acusto-optical-beam-splitter which provides the highest flexibility for setting up the required experimental parameters. This system enables confocal and multiphoton imaging.
Leica TCS SP5 (STED), providing excitation wavelengths of 458, 476, 488, 496, 514, (561) and 633 nm. The system lacks an UV excitation source. The detection system is equipped with for 4 fluorescence channels and one transmission channel. Be aware that the STED depletion laser is removed (no STED, only confocal imaging).
Leica TCS SP8, providing excitation wavelengths of 458, 476, 488, 496, 514, 561 and 633 nm. In addition it has an UV diode laser with excitation at 405 nm. Besides the detection system for 4 fluorescence channels simultaneously, this systems is equipped with an acusto-optical-beam-splitter which provides the highest flexibility for setting up the required experimental parameters. It provides two new Leica HyD hybrid detectors for brilliant imaging with super-sensitivity and contrast and two PMTs.
One super-resolution STED microscope:
Abberior Instruments STED 775 & 595 QUAD Scanning, 3D, providing a multicolor confocal scanning system with two super-resolution STED channels. The system is based on an Olympus IX83 microscope (4-color LED illumination source and a monochrome widefield camera) equipped with the Abberior QUAD scanner (Scan field: >80×80 μm, Line frequency: 2 kHz). The STED system has two pulsed excitation laser sources with wavelengths at 561 and 640 nm and a pulsed STED laser at 775 nm.
For 595 STED the excitation is 485 nm for GFP/YFP-like dyes.
And one motorized research microscope:
Leica DM 6000B, provides brightfield, differential interference, polarization and fluorescence contrast. It is equipped with a 1.4-megapixel, 12-bit monochrome CCD camera.
Specifications of the individual microscopes:
|
Microscope |
Objectives |
Illumination |
Hardware |
Software |
Room |
|
Leica TCS SPE |
Dry: Leica HC PL FLUOTAR 10×/0.30 Leica HCX PL FLUOTAR 40×/0.75 Immersion: Leica HC PL APO 63×/1.40-0.60 OIL |
Laser: 488, 561, 635
Filter cubes: I3 (blue): Exc 450-490, Dichro 510, Suppres LP 515 N2.1 (green): Exc 515-560, Dichro 580, Suppres LP 590 A (UV): Exc 340-380, Dichro 400, Suppres LP 425 BF: Brightfield illumination |
Microscope stand: Leica DM2500 CSQ Detection: PMTs |
Leica LAS AF Version X.X.X.XX |
122 |
|
Leica TCS SP5 |
Dry: Leica HC PL APO CS 10×/0.40 Immersion: Leica HCX PL APO CS 40×/1.25-0.75 OIL Leica HCX PL APO CS 63×/1.40-0.60 OIL Leica HCX PL APO lambda blue 63×/1.40-0.60 OIL UV (for color correction in the blue-green emission range) Leica HCX PL APO CS 63×/1.20 CORR WATER |
UV diode: 405 Argon laser: 458, 476, 488, 496, 514 CW-DPSS laser: 561 HeNe laser:633 |
Microscope stand: Leica DMI 6000 Resonant Scanner Detection: PMTs & APDs |
Leica LAS AF Version 2.7.3.9723 |
122 |
|
Leica TCS SP5 (STED) |
Dry: Leica HC PL APO CS 10×/0.40 Leica HC PL APO CS 20×/0.70 Immersion: Leica HCX PL APO CS 63×/1.20 CORR WATER Leica ACS APO 63×/1.30 OIL Leica HCX PL APO CS STED 100×/1.40 OIL |
Argon laser: 458, 476, 488, 496, 514 CW-DPSS laser: 561 HeNe laser: 633 |
Microscope stand: DMI 6000 Camera: Leica DFC 350FX R2 Detection: PMTs & APDs |
Leica LAS AF Version 2.6.0.7266 |
123 |
|
Leica DM 6000B |
Dry: Leica N PLAN 2.5×/0.07 Leica HC PL FLUOTAR 5×/0.15 Leica HC PL FLUOTAR 10×/0.30 Leica HC PL FLUOTAR 20×/0.50 Leica HCX PL FLUOTAR 40×/0.75 Immersion: Leica HCX PL APO CS 63×/1.20 CORR WATER Leica HCX PL APO 100×/1.40-0.70 OIL |
Mercury Lamp Filter cubes: A4(UV): Exc 360/40, Dichro 400, Suppres BP 470/40 BGR(UV): Exc 420/30;495/15;570/20, Dichro 415;510;590, Suppres BP 465/20;530/30;640/40 GFP(blue): Exc 470/40, Dichro 500, Suppres BP 525/50 L5(blue): Exc BP 480/40, Dichro 505, Suppres LP 527/30 N3(green): Exc BP 546/12, Dichro 565, Suppres LP 600/40 SFRED(red): Exc HQ 630/20x, Dichro Q649LP, Suppres HQ 667/30 |
Microscope stand: Leica DM 6000B Camera: Leica DFC 350FX |
Leica LAS AF Version 2.6.0.7266
AF6000 Modular Systems |
122 |
Abbreviations:
Leica LAS AF = Leica Application Suite Advanced Fluorescence