- Leica LAS AF light (http://www.leica-microsystems.com/products/microscope-software/life-sciences/las-af-advanced-fluorescence/)
Open-Source and Free-Ware
- ImageJ (http://rsb.info.nih.gov/ij/): image processing and analysis, many plugins available.
- FIJI (http://fiji.sc/wiki/index.php/Downloads): Fiji is an image processing package based on ImageJ. Fiji stands for „Fiji is Just ImageJ“. Image processing and analysis, many plugins available (automatic update function). The Bio-Formats plugin is already built in.
- Bio-Formats (http://www.openmicroscopy.org/site/support/bio-formats5/): a standalone Java library for reading and writing life sciences image file formats.
Abberior Instruments Data
The operating system for the Abberior STED microscope is "ImSpector". Imspector stores all files as .msr. Fiji can read .msr files directly without installing any plugin. If you are using ImageJ you need to install the Bio-Formats plugin, which can be downloaded here (http://downloads.openmicroscopy.org/bio-formats/5.0.5). A description on how to install the plugin is given here (http://www.openmicroscopy.org/site/support/bio-formats5/users/imagej/installing.html). Else you can also export each file in ImSpector directly to any file format needed (e.g. .tif).