Live-cell Imaging Facility
The Live-cell Imaging facility provides access to cutting-edge microscopy technologies as well as all-embracing support from sample preparation to assay development to image data analysis – making these services available for all researchers at the institute. The facility assists users in sample preparation by providing reagents and helps develop experimental assays to take advantage of the newest microscopy technologies to image live cells in culture as well as intact embryos, tissues, and organoids. In addition, we provide support in microscope automation (adaptive-feedback/smart microscopy) as well as image and data analysis.
The current setup of the facility includes two high-end confocal systems (Zeiss LSM780 and Zeiss LSM880AiryFast), a STED super-resolution microscope (Aberrior Instruments STEDYCON), and a prototype for iPAM (fast imaging with optical sectioning). The facility also provides rooms for cell culture, microinjection, and sample preparation. For image processing and data analysis the facility has several remotely accessible Virtual Machines (VMs) running on state-of-the-art high performance CPU/GPU servers integrated with >100 TB of storage. Through the VM the facility provides access to a limited number of licenses for Imaris (4D rendering and analysis), Huygens (deconvolution), and open-source software.