Department of Molecular Biology Seminar Series: Genome-wide mapping of protein-DNA interaction dynamics

We recently introduced new tools for high-resolution genome-wide chromatin profiling and have applied them to study chromatin organization and dynamics in a variety of model systems, including yeast, flies and mammals. Among the questions addressed are: How do transcription factors find their binding sites in DNA packaged into nucleosomes? How are nucleosomes depleted from gene regulatory regions? What is the relationship between nucleosome deposition pathways and chromatin deregulation in disease? Our findings illustrate ways in which chromatin dynamics can play a central role in regulating gene expression and silencing.