Fassberg Seminar - ONLINE SEMINAR: Human time vs. mouse time in recapitulated systems

Fassberg Seminar - ONLINE SEMINAR

  • Datum: 16.03.2021
  • Uhrzeit: 11:00 - 12:00
  • Vortragende(r): Miki Ebisuya
  • EMBL Barcelona
  • Ort: Max-Planck-Institut für biophysikalische Chemie (MPIBPC)
  • Raum: Zoom Online Seminar
  • Gastgeber: Marina Rodnina
  • Kontakt: gd.office@mpibpc.mpg.de
Fassberg Seminar - ONLINE SEMINAR: Human time vs. mouse time in recapitulated systems
Different species have different time scales of embryonic development: larger animals tend to grow more slowly than smaller animals. My group has been trying to understand the molecular basis of interspecies differences in developmental time by using in vitro segmentation clock as a model system.
The segmentation clock is the oscillatory gene expressions that regulate the timing of body segment formation from presomitic mesoderm (PSM) during embryogenesis. We have recently succeeded in inducing PSM from both human iPS cells and mouse ES cells, recapitulating the oscillation and traveling wave of segmentation clock in vitro. Interestingly, the oscillation period of human segmentation clock was 5-6 hours while that of mouse was 2-3 hours. Taking advantage of our in vitro system, we measured several biochemical reaction parameters of the core gene of the oscillation mechanism, Hes7, finding out that the degradation and production processes of Hes7 are 2-3 times slower in human PSM cells compared to mouse cells. Our mathematical model quantitatively explained how the slower biochemical reactions in human cells give rise to the longer oscillation period in the human segmentation clock.
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