Emerging Topics in Biomolecular Magnetic Resonance

Presenting frontier research in magnetic resonance in biological systems

Organized by

Loren Andreas, Stefan Glöggler, Christian Griesinger, Mei Hong, Óscar Millet, Arthur G. Palmer, Markus Zweckstetter

Weekly on Thursday

7 a.m. PST (San Francisco) / 10 a.m. EST (New York) / 4 p.m. CST (Berlin) / 7.30 p.m IST (India) / 10 p.m. CST (Peking)

Register here for the Online Seminar

Online Seminar

Thursday, 9^th of June 2022

NMR in Structural Biology – History placed in Perspective

Kurt Wüthrich

Thursday, 2^nd of June 2022

Conformational dynamics of the bacterial oxidoreductase DsbD

Christina Redfield (University of Oxford, UK)

The magic of linking rings

Angela Gronenborn (University of Pittsburgh, USA)

Thursday, 19^th of May 2022

How p14ARF alters the nucleolus; bridging structure, phase separation and tumor suppression

Richard Kriwacki (St Jude Children's Research Hospital, USA)

Protein aggregation and interference with it for treatment studied by NMR and beyond

Christian Griesinger (Max Planck Institute for Multidisciplinary Sciences, Germany)

Thursday, 7^th of May 2022

The dynamics of the Na+/Ca2+ exchanger Ca2+-binding domains investigated by solution NMR spectroscopy

Roberto Salinas (University of São Paulo, Brazil)

Introducing Bayllagio: A Robust Automated Backbone Triple Resonance NMR Assignments of Proteins Using Bayesian-Based Simulated Annealing Bayesian-Based Simulated Annealing

Anthony Bishop (University of Pennsylvania, USA)

Thursday, 21^st of April 2022

NMR-Based High Throughput Screening for Compounds Targeting Single Span Membrane Proteins

Chuck Sanders (Vanderbilt University, USA)

Membrane Protein Unfolding Probed by HDX MAS NMR

Vladimir Ladizhansky (University of Guelph, Canada)

Thursday, 7^thof April 2022

Carbon Detection Sensitivity at High Magnetic Field for Solid-State Protein Structure Determination

Chad Rienstra (University of Wisconsin-Madison, USA)

Visualizing protein breathing motions associated with aromatic ring flipping

Malene Ringkjøbing Jensen (Université Grenoble Alpes, France)

Thursday, 24^th of March 2022

Paramagnetic Metal Ions: From PRE to DNP in Inorganic Solids

Michal Leskes (Weizmann Institute of Science, Israel)

The importance of NMR to comprehend the dynamic world of membrane systems and biocatalysis

Manuel Etzkorn (Heinrich-Heine-University, Germany)

Thursday, 10^th of March 2022

Mapping the transthyretin aggregation landscape by NMR

Peter Wright (The Scripps Research Institute, USA)

Autoinhibition, multivalency, and protein disorder in the regulation of large dynamic molecular machines

Elisar Barbar (Oregon State University, USA)

Thursday, 24^th of February 2022

Conformational disorder in regulation of enzymatic catalysis

Vincenzo Venditti (Iowa State University, USA) more

Structural snapshots in the life cycle of filamentous bacteriophage viruses

Amir Goldbourt (Tel Aviv University, Israel) more

Thursday, 10^th of February 2022

Long-range Conformational Flexibility of the Protein Insertase BamA in the Native Bilayers

Benesh Joseph (Goethe University Frankfurt, Germany) more

Back to Basics: How NMR can help structural biology navigate the era of Big Data and Machine Learning

Jeffrey Hoch (UConn Health, USA) more

Thursday, 27^th of January 2022

From NMR to Translation and Fighting Cancer

Gerhard Wagner (Harvard Medical School, USA)

Amyloid Protein-aggregation and -interactions by EPR

Martina Huber (Leiden University, Netherlands)
more

Thursday, 13^thof January 2022

NMR experiments based on 13C direct detection reveal novel motifs in IDPs

Isabella Felli (CERM, Italy)
more

NMR investigation of dimerization of cadherin-11 and New approaches for analysis of spin relaxation data

Arthur G. Palmer 3rd (Columbia University, USA)
more

Thursday, 9^thof December 2021

Integrative (semi-)structural biology that utilizes distribution restraints

Gunnar Jeschke (ETH Zürich, Switzerland)

Liquid state protein NMR to study molecular crowding

Jochen Balbach (Martin-Luther-Universität Halle-Wittenberg, Germany)

Thursday, 25^thof November 2021

Protein Folding on the Ribosome

John Christodoulou (University College London, UK)

Adventures in Biomolecular NMR with para-hydrogen

Stefan Glöggler (Max Planck Institute for Biophysical Chemistry, Germany)

Thursday, 5^th of August 2021

Designer peptide co-assembly and what it may be teaching us about naturally occurring protein aggregation

Anant Paravastu (Georgia Institute of Technology)

Revealing the role of arginine methylation as key regulator of disordered proteins

Tobias Madl (Medical University of Graz)

Thursday, 29^th of July 2021

Investigating a-Synuclein amyloid seeding processes by NMR

Jean Baum (Rutgers University)

Higher fields and faster spinning: new opportunities for protein NMR

Beat Meier (ETH Zürich)

Thursday, 22^nd of July 2021

NMR studies of the HIV-1 RNA Packaging Signal

Michael F. Summers (University of Maryland)

The amyloid structure of RIPK3 (receptor interacting protein kinase 3) of mouse and human in cell necroptosis

Jun-Xia Lu (ShanghaiTech University)

Thursday, 15^th of July 2021

Solution NMR of phase separated disordered proteins associated with cancer and neurodegeneration

Nicolas Fawzi (Brown University)

Drugs in Cells

Lyndon Emsely (École Polytechnique Fédérale de Lausanne)

Thursday, 1^st of July 2021

Hyperpolarized water with dissolution-DNP and UV radicals

Jan Henrik Ardenkjær-Larsen (Technical University of Denmark) more

De novo structure determination of organic compounds from multi-alignment datasets: chances and challenges

Christina Thiele (Technical University of Darmstadt) more

Thursday, 10^th of June 2021

Peptide-mediated lipid organization, structure, and dynamics in pulmonary surfactant

Joanna Long (University of Florida/Florida State University)

Quantitative Protein Order/Disorder Assessment Using NMR Chemical Shifts

Frans Mulder (Aarhus University)

Thursday, 3^rd of June 2021

19F NMR Inspired Studies of GPCR Activation and the Connection to Pharmacology - A Non-Wikipedia Perspective

Scott Prosser (University of Toronto)

NMR spectroscopy applied to drug discovery and in metabolism characterization

Óscar Millet (CIC bioGUNE)

Thursday, 27^th of May 2021

Characterization of Biotherapeutics by Chemometric Analysis of NMR Spectra

Frank Delaglio (NIST & University of Maryland) more

NMR detection of light-dependent processes in membrane

Clemens Glaubitz (Goethe-Universitat Frankfurt am Main) more

Thursday, 20^th of May 2021

Comparative assessment of protein higher order structure in biopharmaceutical development using NMR and chemometric analysis

Hai-Young Kim (Pfizer, Inc.) more

Detection of a bound water molecule proton in a membrane protein pore

Loren Andreas (Max Planck Institute for Biophysical Chemistry) more

Thursday, 13^th of May 2021

Capturing the high-resolution structure of a low-populated aromatic ring flipping intermediate

Malene Ringkjøbing Jensen (Institut de Biologie Structurale, Grenoble) more

Protein folding and misfolding at the membrane surface

Alfonso De Simone (University of Naples Federico II) more

Thursday, 6^th of May 2021

An insight into stem cell metabolism with NMR metabolomics

Ana Gil (University of Aveiro)

The RNA binding, droplet formation and disease mechanism of FUS as probed by NMR

Frédéric Allain (ETH Zürich) more

Thursday, 29th of April 2021

The outer membrane insertase BAM as an antibiotic target

Sebastian Hiller (University of Basel) more

Relaxation dispersion under fast magic-angel spinning

Petra Rovó (Ludwig Maximilians University Munich) more

Thursday, 22^nd of April 2021

Investigation of the lipidated C-terminus of RalA: mutually exclusive binding to membranes and calmodulin

Helen Mott (University of Cambridge) more

SHACA, novel universal rotation pulses, and orientational constraints: tools and concepts for bio-NMR

Burkhard Luy (Karlsruhe Institute of Technology) more

Thursday, 15^th of April 2021

Probing submillisecond, pre-nucleation oligomerization of huntingtin exon-1 by NMR

Marius Clore (NIDDK, NIH) more

Structural dynamics of membrane proteins by NMR with fast MAS

Guido Pintacuda (Université de Lyon) more

Thursday, 8^th of April 2021

In-cell EPR: latest developments for membrane proteins

Enrica Bordignon (Ruhr University Bochum)

Filling the Knowledge Gap of the HIV-1 Spike Structure and Implication to Vaccine Development

James Chou (Harvard Medical School) more

Thursday, 1^st of April 2021

Helix-helix interactions of receptor tyrosine kinases

Anne Ulrich (Karlsruhe Institute of Technology) more

Fast assignment of protein hotspots with Focused Spectroscopy (FOSY)

Vladislav Orekhov (University of Gothenburg) more

Thursday, 25^th of March 2021

In-cell NMR: a powerful approach for drug and fragment screening

Lucia Banci (CERM/University of Florence) more

Interactions between small molecules and Aβ amyloid fibrils probed by MAS solid-state NMR

Bernd Reif (Technical University Munich) more

Thursday, 18^th of March 2021

Paramagnetic probes for NMR and EPR: Lock up the metals!

Marcellus Ubbink (Universiteit Leiden) more

Integrated Structural Biology of HIV-1 Assemblies

Tatyana Polenova (University of Delaware) more

Thursday, 11^th of March 2021

Copper Ions and Protein Aggregation: From Amyloids in Diabetes to Non-Amyloids in Cataracts Disease

Liliana Quintanar (Cinvestav, Mexico) more

Methyl resonance assignments, from methyl NOEs: Fact or Fiction?

Nikolaos Sgourakis (University of Pennsylvania) more

Thursday, 4^th of March 2021

Disordered complexes involving highly charged proteins

Birthe Kragelund (University of Copenhagen)

New Insights in COVID-19 from NMR Metabolomics

Ulrich Günther (University of Lübeck) more

Thursday, 25^th of February 2021

Putting annoying chemical exchanges to good use in the solution-state NMR of sugars, proteins and nucleic acids

Lucio Frydman (Weizmann Institute) more

Measuring the accuracy of NMR protein structures

Mike Williamson (University of Sheffield) more

Thursday, 18^th of February 2021

On automated structure and multi-state structure determination by NMR

Roland Riek (ETH Zürich) more

SSNMR-detected mobility in substrate-mediated protein activation

Patrick van der Wel (University of Groningen) more

Thursday, 11^th of February 2021

The conformational landscape of protein kinases in physiology and disease

Babis Kalodimos (St. Jude Children’s Research Hospital) more

Additive fabrication methods for reproducible, open-source NMR instrumentation

Rachel Martin (University of California, Irvine) more

Thursday, 4^th of February 2021

Understanding the molecular interactions underlying liquid-liquid phase separation of low-complexity domains

Tanja Mittag (St. Jude Children’s Research Hospital) more

Atomic structures and functional dynamics of rhomboid proteases, non-selective cation channels, and bacteriophage tail tubes

Adam Lange (Humboldt-University Berlin) more

Thursday, 28^th of January 2021

Molecular insights to calcified deposit formation from NMR

Francesca Marassi (Sanford Burnham Prebys) more

Breaking down the mechanism of a proton-coupled transporter

Katherine Henzler-Wildman (UW-Madison/NMRFAM) more

Thursday, 21^th of January 2021

Membrane Interactions of 40-Residue Beta-Amyloid Peptides

Wei Qiang (Binghamton University) more

1H-detected solid-state NMR as an access to µs timescale conformational dynamics in large enzymes

¹H-detected solid-state NMR as an access to µs timescale conformational dynamics in large enzymes

Rasmus Linser (Technical University Dortmund) more

Thursday, 14^th of January 2021

Transient structure in IDPs from 3D NUS NMR of chemical denaturation

Ad Bax (NIDDK/NIH) more

Time Travel to the Past and Future – Evolution of energy landscapes for enzymes catalysis

Dorothee Kern (Brandeis University) more

Thursday, 10^th of December 2020

Structures of SARS-CoV-2 & Influenza Viroporins: a Quest to Block Pandemics

Mei Hong (MIT, Cambridge MA) more

Biomolecular condensation: from transcription to SARS-CoV-2

Markus Zweckstetter (DZNE/MPIBPC, Göttingen) more

Thursday, 3^rd of December 2020

Nanoparticle-assisted NMR spin relaxation: a new window into biomolecular interactions and dynamics

Rafael Brüschweiler (The Ohio State University)

Tracking the evolution of tau in solution, droplet and fibril states

Songi Han (UC Santa Barbara)

Thursday, 26^th of November 2020

Just flexible linkers? Un-structural biology by NMR

Roberta Pierattelli (University of Florence/CERM)

Factor X interaction with Ixolaris reveals a noncanonical mechanism of Kunitz inhibition

Ana Paula Valente (Federal University of Rio de Janeiro)

Thursday, 19^th of November 2020

ALIX in Wonderland: Multivalency and Phosphorylation-mediated Reversible Amyloids Studied by Solution NMR Spectroscopy

Lalit Deshmukh (University of California San Diego)

Lipid regulation of a K+ channel viewed by solid-state NMR

Benjamin J. Wylie (Texas Tech University)

Thursday, 12^th of November 2020

High-resulution NMR ob biomulecules, large and small, over orders of magnitude of magnetic fields

Fabien Ferrage (CNRS) more

Solid-state NMR approaches for cellular structural biology

Marc Baldus (Utrecht University) more

Thursday, 5^th of November 2020

Eavesdropping on the Cellular Adventures of a Pathogenic Cytotoxin

Jordan Chill (Bar Ilan University) more

Integrated Structural Biology of HIV-1 Assemblies

Tatyana Polenova (University of Delaware)

Thursday, 29^th of October 2020

Structure and function of biomolecules by detecting nuclear spins with EPR

Marina Bennati (Max Planck Institute for Biophysical Chemistry)

Mechanisms of Action for Pathogenic Mutations in the FGF Receptor Kinase

Nathaniel Traaseth (New York Frankfurt) more

Thursday, 22^nd of October 2020

Dynamic regulation of client and cochaperone interactions of Hsp90

Michael Sattler (Helmholtz Center & Technical University Munich)

Time-resolved solid state NMR of biomolecular self-assembly processes

Robert Tycko (NIDDK, National Institutes of Health) more

Thursday, 15^thof October 2020

Turning peptide toxins into therapeutics: the importance of structure AND dynamics

Raymond Norton (Monash University)

Protein (mis)folding at high sensitivity and resolution

Henrike Heise (Forschungszentrum Jülich) more

Thursday, 5^th of October 2020

Beyond static structures: Linking conformational changes in enzymes with function

Remco Sprangers (University of Regensburg) more

Innovations in Protein SSNMR using Ultra-fast MAS and SSNMR Studies on New Polymorphs of Ab42 Fibrils

Yoshitaka Ishii (Tokyo Institute of Technology & RIKEN)

Thursday, 1^st of October 2020

Interactions and dynamics of arginine side chains in proteins by 13C-detected NMR

Interactions and dynamics of arginine side chains in proteins by ¹³C-detected NMR

Flemming D. Hansen (University College London) more

Structural architecture of yeast cell wall and functional amyloids by solid-state NMR

Antoine Loquet (Universite Bordeaux) more

Thursday, 24^th of September 2020

Finding order in disordered proteins

Robert Konrat (University of Vienna) more

The conformational equilibrium of the neuropeptide Y2 G protein-coupled receptor in membranes studied by solid-state NMR

Daniel Huster (Leipzig University) more

THURSDAY, 17^th of September 2020

Regulins control Ca2+-ATPase function via topological allostery

Regulins control Ca²⁺-ATPase function via topological allostery

Gianluigi Veglia (University of Minnesota) more

Unveiling the “invisible” regulatory states in RNA

Qi Zhang (University of North Carolina at Chapel Hill)

THURSDAY, 10^th of September 2020

Proton detected experiments for structural characterization of biomolecules and a-synuclin fibril polymorphs from different aggregation intermediates

Vipin Agarwal (TIFR Hyderabad)

NMR-guided fragment-to-lead evolution

Ruan Ke (University of Science & Technology of China) more

THURSDAY, 3^rd of September 2020

Proton-detected solid-state NMR of viral protein assemblies

Anja Böckmann (University Lyon) more

New CEST Experiments to study protein conformational exchange

Pramodh Vallurupalli (Tata Institute of Fundamental Research) more

THURSDAY, 27^th of August 2020

NMR characterization of the structural E and S membrane proteins of SARS-CoV-2

Sang Ho Park (University of California, San Diego) more

NMR studies of SARS-CoV-2 viral RNAs, proteins and their complexes and screening against viral targets

Andreas Schlundt/Boris Fürtig (University Frankfurt) more

THURSDAY, 20^th of August 2020

Old wine in a new bottle: Repurposing 19F-NMR for biomolecules by combating the deleterious effects of CSA with TROSY

Haribabu Arthanari (Harvard Medical School) more

Resolving structure and functional dynamics of large protein complexes by solid-state NMR, cryo-EM and MD: from new methods to biological insight

Paul Schanda (Institut de Biologie Structurale, Grenoble) more

THURSDAY, 13^th of August 2020

Structural rearrangement modulates the regulatory activity in CCCH-type zinc finger proteins

Francesca Massi (University of Massachusetts) more

Heteronuclear spin decoupling for all (commerical) MAS frequencies: Methods and recipes

P. K. Madhu (TIFR Hyderabad) more

THURSDAY, 6^th of August 2020

The mystery of the double peak in the EPR spectrum of transferrin

Guinevere Mathies (University of Konstanz) more

High pressure NMR: Squeezing out unique information on protein ligand binding and conformational changes

Kevin Gardner (CUNY Advanced Science Research Center, New York) more

THURSDAY, 30^th of July 2020

MicroRNA dynamics by NMR: excited to see the invisible

Katja Petzold (Karolinska Institute) more

Novel nanodiscs for NMR studies on membrane proteins

Ayyalusamy Ramamoorthy (University of Michigan) more

THURSDAY, 23^rd of July 2020

High-resolution NMR of antibiotics in cell membranes

Markus Weingarth (Utrecht University) more

NMR of highly disordered molecular assemblies involved in viral replication

Martin Blackledge (Institut de Biologie Structurale Jean-Pierre Ebel, CEA, CNRS) more

THURSDAY, 16^th of July 2020

Solution NMR insights into phase separation of intrinsically disordered protein regions

Julie Forman-Kay (University of Toronto) more

MAS NMR studies of heterochromatin phase separation

Galia Debelouchina (University of California, San Diego)

THURSDAY, 9^th of July 2020

Spin state evolution during the biological water splitting reaction

Nick Cox (Australian National University) more

In cell structural biology enabled by DNP MAS NMR

Kendra Frederick (UT Southwestern) more

THURSDAY, 2^nd of July 2020

Structural equilibrium underlying ligand-dependent activation of GPCR

Ichio Shimada (RIKEN Center for Biosystems Dynamics Research)

A high-resolution description of β1-adrenergic receptor functional dynamics and allosteric coupling from backbone NMR

Anne Grahl (Biozentrum, University of Basel) more

THURSDAY, 25^th of June 2020

Understanding extracellular matrix disease states with solid-state NMR?

Melinda J. Duer (University of Cambridge) more

NMR for metabolomics: again the 'second best' technique?

Claudio Luchinat (University of Florence) more

THURSDAY, 18^th of June 2020

Elucidation of carbohydrate structure in plant biomass and fungal pathogens using solid-state NMR and DNP methods

Tuo Wang (Lousiana State University) more

Molecular chaperones in protein disaggregation - What can we learn from NMR

Rina Rosenzweig (Weizmann Institute of Sciencey)

THURSDAY, 11^th of June 2020

Putative Amyloids in Human Health— Insights from NMR

Ann E. McDermott (Columbia University) more

An RNA dynamic ensemble at atomic resolution

Hashim Al-Hashimi (Duke University) more

THURSDAY, 4^th of June 2020

Mn(II) DEER tracks two ‘closed’ ATP-, ADP-dependent conformations in yeast Hsp90 chaperone

Daniella Goldfarb (Weizmann Institute of Science) more

NMR: The Big and the Invisible

Lewis Kay (University of Toronto) more

Emerging Topics in Biomolecular Magnetic Resonance

Online Seminar

Thursday, 9th of June 2022

NMR in Structural Biology – History placed in Perspective

Thursday, 2nd of June 2022

Conformational dynamics of the bacterial oxidoreductase DsbD

The magic of linking rings

Thursday, 19th of May 2022

How p14ARF alters the nucleolus; bridging structure, phase separation and tumor suppression

Protein aggregation and interference with it for treatment studied by NMR and beyond

Thursday, 7th of May 2022

The dynamics of the Na+/Ca2+ exchanger Ca2+-binding domains investigated by solution NMR spectroscopy

Introducing Bayllagio: A Robust Automated Backbone Triple Resonance NMR Assignments of Proteins Using Bayesian-Based Simulated Annealing Bayesian-Based Simulated Annealing

Thursday, 21st of April 2022

NMR-Based High Throughput Screening for Compounds Targeting Single Span Membrane Proteins

Membrane Protein Unfolding Probed by HDX MAS NMR

Thursday, 7th of April 2022

Carbon Detection Sensitivity at High Magnetic Field for Solid-State Protein Structure Determination

Visualizing protein breathing motions associated with aromatic ring flipping

Thursday, 24th of March 2022

Paramagnetic Metal Ions: From PRE to DNP in Inorganic Solids

The importance of NMR to comprehend the dynamic world of membrane systems and biocatalysis

Thursday, 10th of March 2022

Mapping the transthyretin aggregation landscape by NMR

Autoinhibition, multivalency, and protein disorder in the regulation of large dynamic molecular machines

Thursday, 24th of February 2022

Thursday, 10th of February 2022

Thursday, 27th of January 2022

From NMR to Translation and Fighting Cancer

Thursday, 13th of January 2022

Thursday, 9th of December 2021