MPI Campus Seminar: Detecting water molecules at radical intermediates in enzymatic catalysis

MPI Campus Seminar

  • Datum: 24.02.2021
  • Uhrzeit: 11:00 - 12:00
  • Vortragende(r): Fabian Hecker
  • Research Group Electron Paramagnetic Resonance
  • Ort: Max-Planck-Institut für biophysikalische Chemie (MPIBPC)
  • Raum: Online
  • Gastgeber: S. Glöggler, A. Godec, A. Faesen, J. Liepe, S. Meek, A. Stein, M. Wilczek, S. Karpitschka, D. Zwicker, M. Oudelaar, L. Andreas
  • Kontakt: stefan.gloeggler@mpibpc.mpg.de
MPI Campus Seminar: Detecting water molecules at radical intermediates in enzymatic catalysis
The role of water in biological processes and reactions is highly divers and reaches far beyond that of a mere solvent. Of particular interest is the involvement of individual water molecules in electron-transfer processes, in which it may take the role of proton acceptor, donor or simply stabilizer. The identification of internal water in proteins can be achieved by X-ray diffraction however crystallization of transient protein complexes is difficult. A key approach to detect water in protein complexes is the use of 17O-enriched water in conjunction with magnetic resonance spectroscopy. Electron paramagnetic resonance (EPR) is particularly suited, since it can selectively detect nuclei in the coordination sphere of paramagnetic centers, such as radical intermediates. In this project, we use electron nuclear double resonance spectroscopy (ENDOR) to reveal the fingerprint of water molecules at three trapped radicals in the active protein complex of E. coli ribonucleotide reductase.
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